|Application ||WB, IHC-P, E|
|Calculated MW||111694 Da|
|Antigen Region||239-268 aa|
|Other Names||Ubiquitin-like modifier-activating enzyme 7, Ubiquitin-activating enzyme 7, D8, Ubiquitin-activating enzyme E1 homolog, UBA7, UBE1L, UBE2|
|Target/Specificity||This UBA7 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 239-268 amino acids from the N-terminal region of human UBA7.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||UBA7 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Activates ubiquitin by first adenylating with ATP its C- terminal glycine residue and thereafter linking this residue to the side chain of a cysteine residue in E1, yielding a ubiquitin- E1 thioester and free AMP. Catalyzes the ISGylation of influenza A virus NS1 protein.|
|Tissue Location||Expressed in a variety of normal and tumor cell types, but is reduced in lung cancer cell lines|
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Provided below are standard protocols that you may find useful for product applications.
The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E1 ubiquitin-activating enzyme family. The encoded enzyme is a retinoid target that triggers promyelocytic leukemia (PML)/retinoic acid receptor alpha (RARalpha) degradation and apoptosis in acute promyelocytic leukemia, where it is involved in the conjugation of the ubiquitin-like interferon-stimulated gene 15 protein. [provided by RefSeq].
Fransen, K., et al. Hum. Mol. Genet. 19(17):3482-3488(2010)
Morgan, A.R., et al. Hum. Immunol. 71(6):602-609(2010)
Feng, Q., et al. Mol. Cancer Ther. 7(12):3780-3788(2008)
Durfee, L.A., et al. J. Biol. Chem. 283(35):23895-23902(2008)
Takeuchi, T., et al. J. Biochem. 138(6):711-719(2005)
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