|Application ||WB, IHC-P, E|
|Other Accession||P70583, NP_001939.1, NP_001020419.1, NP_001020420.1|
|Calculated MW||26563 Da|
|Antigen Region||170-198 aa|
|Other Names||Deoxyuridine 5'-triphosphate nucleotidohydrolase, mitochondrial, dUTPase, dUTP pyrophosphatase, DUT|
|Target/Specificity||This DUT antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 170-198 amino acids from the C-terminal region of human DUT.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||DUT Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||This enzyme is involved in nucleotide metabolism: it produces dUMP, the immediate precursor of thymidine nucleotides and it decreases the intracellular concentration of dUTP so that uracil cannot be incorporated into DNA.|
|Cellular Location||Isoform 2: Nucleus.|
|Tissue Location||Found in a variety of tissues. Isoform 3 expression is constitutive, while isoform 2 expression correlates with the onset of DNA replication (at protein level). Isoform 2 degradation coincides with the cessation of nuclear DNA replication (at protein level).|
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Provided below are standard protocols that you may find useful for product applications.
This gene encodes an essential enzyme of nucleotide metabolism. The encoded protein forms a ubiquitous, homotetrameric enzyme that hydrolyzes dUTP to dUMP and pyrophosphate. This reaction serves two cellular purposes: providing a precursor (dUMP) for the synthesis of thymine nucleotides needed for DNA replication, and limiting intracellular pools of dUTP. Elevated levels of dUTP lead to increased incorporation of uracil into DNA, which induces extensive excision repair mediated by uracil glycosylase. This repair process, resulting in the removal and reincorporation of dUTP, is self-defeating and leads to DNA fragmentation and cell death. Alternative splicing of this gene leads to different isoforms that localize to either the mitochondrion or nucleus. A related pseudogene is located on chromosome 19.
Takatori, H., et al. Liver Int. 30(3):438-446(2010) Quesada-Soriano, I., et al. Biochimie 92(2):178-186(2010) Chanson, A., et al. Am. J. Clin. Nutr. 89(6):1927-1936(2009) Takacs, E., et al. FEBS Lett. 583(5):865-871(2009) Venkatesan, K., et al. Nat. Methods 6(1):83-90(2009)
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