|Application ||WB, E|
|Calculated MW||64507 Da|
|Antigen Region||472-500 aa|
|Other Names||Frizzled-5, Fz-5, hFz5, FzE5, FZD5, C2orf31|
|Target/Specificity||This FZD5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 472-500 amino acids from the C-terminal region of human FZD5.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||FZD5 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Receptor for Wnt proteins. Most of frizzled receptors are coupled to the beta-catenin canonical signaling pathway, which leads to the activation of disheveled proteins, inhibition of GSK- 3 kinase, nuclear accumulation of beta-catenin and activation of Wnt target genes. A second signaling pathway involving PKC and calcium fluxes has been seen for some family members, but it is not yet clear if it represents a distinct pathway or if it can be integrated in the canonical pathway, as PKC seems to be required for Wnt-mediated inactivation of GSK-3 kinase. Both pathways seem to involve interactions with G-proteins. May be involved in transduction and intercellular transmission of polarity information during tissue morphogenesis and/or in differentiated tissues. Interacts specifically with Wnt5A to induce the beta- catenin pathway.|
|Cellular Location||Cell membrane; Multi-pass membrane protein. Golgi apparatus membrane; Multi-pass membrane protein Note=Localized at the plasma membrane and also found at the Golgi|
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Provided below are standard protocols that you may find useful for product applications.
Members of the 'frizzled' gene family encode 7-transmembrane domain proteins that are receptors for Wnt signaling proteins. The FZD5 protein is believed to be the receptor for the Wnt5A ligand.
Moller, M., et al. BMC Infect. Dis. 10, 154 (2010) :
Yerges, L.M., et al. J. Bone Miner. Res. 24(12):2039-2049(2009)
Terabayashi, T., et al. J. Biol. Chem. 284(39):26716-26724(2009)
Kim, J.G., et al. J. Korean Med. Sci. 24(3):443-447(2009)
Lin, Z., et al. J. Biol. Chem. 283(48):33053-33058(2008)
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