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Mouse Phkg1 Antibody (C-term)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - Mouse Phkg1 Antibody (C-term) AP14255b
    Mouse Phkg1 Antibody (C-term) (Cat. #AP14255b) western blot analysis in Ramos cell line lysates (35ug/lane).This demonstrates the Phkg1 antibody detected the Phkg1 protein (arrow).
    detail
  • WB - Mouse Phkg1 Antibody (C-term) AP14255b
    Mouse Phkg1 Antibody (C-term) (Cat. #AP14255b) western blot analysis in mouse stomach tissue lysates (35ug/lane).This demonstrates the Phkg1 antibody detected the Phkg1 protein (arrow).
    detail
  • FC - Mouse Phkg1 Antibody (C-term) AP14255b
    Mouse Phkg1 Antibody (C-term) (Cat. #AP14255b) flow cytometric analysis of Ramos cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
FC, WB, E
Primary Accession P07934
Other Accession NP_035209.1
Reactivity Human, Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 44960 Da
Antigen Region 302-331 aa
Additional Information
Gene ID 18682
Other Names Phosphorylase b kinase gamma catalytic chain, skeletal muscle/heart isoform, Phosphorylase kinase subunit gamma-1, Serine/threonine-protein kinase PHKG1, Phkg1, Phkg
Target/Specificity This Mouse Phkg1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 302-331 amino acids from the C-terminal region of mouse Phkg1.
Dilution WB~~1:1000
FC~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsMouse Phkg1 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name Phkg1
Synonyms Phkg
Function Catalytic subunit of the phosphorylase b kinase (PHK), which mediates the neural and hormonal regulation of glycogen breakdown (glycogenolysis) by phosphorylating and thereby activating glycogen phosphorylase. In vitro, phosphorylates PYGM, TNNI3, MAPT/TAU, GAP43 and NRGN/RC3 (By similarity).
Research Areas
Citations (0)
citation

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Background

Phosphorylase b kinase catalyzes the phosphorylation of serine in certain substrates, including troponin I.

References

Suto, J., et al. J. Vet. Med. Sci. 66(9):1033-1038(2004)
Visel, A., et al. Nucleic Acids Res. 32 (DATABASE ISSUE), D552-D556 (2004) :
Takenaka, M., et al. Kidney Int. 53(3):562-572(1998)
Maichele, A.J., et al. Mamm. Genome 5(1):15-18(1994)
Bender, P.K., et al. Genet. Anal. Tech. Appl. 10 (3-4), 99-101 (1993) :

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$ 365.00
$ 140.00
Cat# AP14255b
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