|Application ||WB, IHC-P, E|
|Other Accession||Q32P79, NP_006225.1|
|Calculated MW||13293 Da|
|Antigen Region||89-117 aa|
|Other Names||DNA-directed RNA polymerase II subunit RPB11-a, RNA polymerase II subunit B11-a, RPB11a, DNA-directed RNA polymerase II subunit J-1, RNA polymerase II 133 kDa subunit, POLR2J, POLR2J1|
|Target/Specificity||This POLR2J antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 89-117 amino acids from the C-terminal region of human POLR2J.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||POLR2J Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Component of RNA polymerase II which synthesizes mRNA precursors and many functional non-coding RNAs. Pol II is the central component of the basal RNA polymerase II transcription machinery. It is composed of mobile elements that move relative to each other. RPB11 is part of the core element with the central large cleft (By similarity).|
|Tissue Location||Ubiquitously expressed. High expression was found in heart and skeletal muscle.|
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Provided below are standard protocols that you may find useful for product applications.
This gene encodes a subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. The product of this gene exists as a heterodimer with another polymerase subunit; together they form a core subassembly unit of the polymerase. Two similar genes are located nearby on chromosome 7q22.1 and a pseudogene is found on chromosome 7p13.
Michiels, S., et al. Carcinogenesis 30(5):763-768(2009)
Oh, J.H., et al. Mamm. Genome 16(12):942-954(2005)
Shpakovskii, D.G., et al. Bioorg. Khim. 30(6):621-625(2004)
Zhou, M., et al. Proc. Natl. Acad. Sci. U.S.A. 100(22):12666-12671(2003)
Kaehlcke, K., et al. Mol. Cell 12(1):167-176(2003)
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