Mouse Irak3 Antibody (C-term)
Affinity Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application
| WB, E |
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Primary Accession | Q8K4B2 |
Other Accession | NP_082955.2 |
Reactivity | Mouse |
Host | Rabbit |
Clonality | Polyclonal |
Isotype | Rabbit IgG |
Calculated MW | 68455 Da |
Antigen Region | 517-544 aa |
Gene ID | 73914 |
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Other Names | Interleukin-1 receptor-associated kinase 3, IRAK-3, IL-1 receptor-associated kinase M, IRAK-M, Irak3 {ECO:0000312|MGI:MGI:1921164} |
Target/Specificity | This Mouse Irak3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 517-544 amino acids from the C-terminal region of mouse Irak3. |
Dilution | WB~~1:1000 |
Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Precautions | Mouse Irak3 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures. |
Name | Irak3 {ECO:0000312|MGI:MGI:1921164} |
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Function | Putative inactive protein kinase which regulates signaling downstream of immune receptors including IL1R and Toll-like receptors (PubMed:12150927, PubMed:12054681, PubMed:29686383). Inhibits dissociation of IRAK1 and IRAK4 from the Toll-like receptor signaling complex by either inhibiting the phosphorylation of IRAK1 and IRAK4 or stabilizing the receptor complex (PubMed:12150927, PubMed:12054681). Upon IL33-induced lung inflammation, positively regulates expression of IL6, CSF3, CXCL2 and CCL5 mRNAs in dendritic cells (PubMed:29686383). |
Cellular Location | Cytoplasm. Nucleus. Note=In dendritic cells, translocates into the nucleus upon IL33 stimulation. |
Tissue Location | Expressed in inflamed lung macrophages (at protein level) (PubMed:29686383). Expressed in dendritic cells (at protein level) (PubMed:29686383). Highly expressed in liver and thymus and at lower levels in heart, brain, spleen and kidney (PubMed:12054681) |
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Provided below are standard protocols that you may find useful for product applications.
Background
Irak3 inhibits dissociation of IRAK1 and IRAK4 from the Toll-like receptor signaling complex by either inhibiting the phosphorylation of IRAK1 and IRAK4 or stabilizing the receptor complex.
References
Turnis, M.E., et al. J. Immunol. 185(7):4223-4232(2010)
Hubbard, L.L., et al. J. Immunol. 184(11):6299-6308(2010)
Seki, M., et al. J. Immunol. 184(3):1410-1418(2010)
Lyn-Kew, K., et al. PLoS ONE 5 (6), E11145 (2010) :
Su, J., et al. J Innate Immun 1(2):164-174(2009)
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