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APG10L Antibody (C-term S116)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

  • WB - APG10L Antibody (C-term S116) AP14315c
    APG10L Antibody (C-term S116) (Cat. #AP14315c) western blot analysis in NCI-H292 cell line lysates (35ug/lane).This demonstrates the APG10L antibody detected the APG10L protein (arrow).
  • WB - APG10L Antibody (C-term S116) AP14315c
    Western blot analysis of APG10L (arrow) using rabbit polyclonal APG10L Antibody (C-term S116) (Cat. #AP14315c). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the APG10L gene.
  • IHC-P - APG10L Antibody (C-term S116) AP14315c
    APG10L Antibody (C-term S116) (Cat. #AP14315c)immunohistochemistry analysis in formalin fixed and paraffin embedded human rectum tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of ANKS1B APG10L Antibody (C-term S116) for immunohistochemistry. Clinical relevance has not been evaluated.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q9H0Y0
Other Accession Q8R1P4, NP_001124500.1
Reactivity Human
Predicted Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 25279 Da
Antigen Region 96-121 aa
Additional Information
Gene ID 83734
Other Names Ubiquitin-like-conjugating enzyme ATG10, 632-, Autophagy-related protein 10, APG10-like, ATG10, APG10L
Target/Specificity This APG10L antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 96-121 amino acids from the C-terminal region of human APG10L.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsAPG10L Antibody (C-term S116) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name ATG10
Synonyms APG10L
Function E2-like enzyme involved in autophagy. Acts as an E2-like enzyme that catalyzes the conjugation of ATG12 to ATG5. ATG12 conjugation to ATG5 is required for autophagy. Likely serves as an ATG5-recognition molecule. Not involved in ATG12 conjugation to ATG3 (By similarity). Plays a role in adenovirus-mediated cell lysis.
Cellular Location Cytoplasm.
Research Areas
Citations (0)

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Autophagy is a process for the bulk degradation of cytosolic compartments by lysosomes. ATG10 is an E2-like enzyme involved in 2 ubiquitin-like modifications essential for autophagosome formation: ATG12 (MIM 609608)-ATG5 (MIM 604261) conjugation and modification of a soluble form of MAP-LC3 (MAP1LC3A; MIM 601242), a homolog of yeast Apg8, to a membrane-bound form (Nemoto et al., 2003 [PubMed 12890687]).


Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010) :
Criollo, A., et al. Cell Death Differ. 14(5):1029-1039(2007)
Shao, Y., et al. Autophagy 3(1):10-16(2007)
Ewing, R.M., et al. Mol. Syst. Biol. 3, 89 (2007) :
Boya, P., et al. Mol. Cell. Biol. 25(3):1025-1040(2005)

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$ 295.00
$ 99.00
Cat# AP14315c
(40 western blots)
Availability: In Stock
Bulk Size
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