|Application ||WB, IHC-P, E|
|Other Accession||Q8R1P4, NP_001124500.1|
|Calculated MW||25279 Da|
|Antigen Region||96-121 aa|
|Other Names||Ubiquitin-like-conjugating enzyme ATG10, 632-, Autophagy-related protein 10, APG10-like, ATG10, APG10L|
|Target/Specificity||This APG10L antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 96-121 amino acids from the C-terminal region of human APG10L.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||APG10L Antibody (C-term S116) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||E2-like enzyme involved in autophagy. Acts as an E2-like enzyme that catalyzes the conjugation of ATG12 to ATG5. ATG12 conjugation to ATG5 is required for autophagy. Likely serves as an ATG5-recognition molecule. Not involved in ATG12 conjugation to ATG3 (By similarity). Plays a role in adenovirus-mediated cell lysis.|
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Provided below are standard protocols that you may find useful for product applications.
Autophagy is a process for the bulk degradation of cytosolic compartments by lysosomes. ATG10 is an E2-like enzyme involved in 2 ubiquitin-like modifications essential for autophagosome formation: ATG12 (MIM 609608)-ATG5 (MIM 604261) conjugation and modification of a soluble form of MAP-LC3 (MAP1LC3A; MIM 601242), a homolog of yeast Apg8, to a membrane-bound form (Nemoto et al., 2003 [PubMed 12890687]).
Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010) :
Criollo, A., et al. Cell Death Differ. 14(5):1029-1039(2007)
Shao, Y., et al. Autophagy 3(1):10-16(2007)
Ewing, R.M., et al. Mol. Syst. Biol. 3, 89 (2007) :
Boya, P., et al. Mol. Cell. Biol. 25(3):1025-1040(2005)
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