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APG10L Antibody (C-term S116)Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
| Country | United States
Ordering Information
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| Catalog # | Size | Availability | Price | |
| AP14315c | 0.1 mg 400 ul | In Stock | $ 255.00 | DISCONTINED INQUIRE CLICK INQUIRE Add to cart |
| AP14315c-ev20 | 20 ug 100 ul | In Stock | $ 95.00 | DISCONTINED INQUIRE CLICK INQUIRE Add to cart |
- Specification
- Citiations : 0
- Reviews
- Protocols
- Backgrounds
APG10L Antibody (C-term S116) - Product info | |
| Application | WB, IHC
|
| Primary Accession | Q9H0Y0 |
| Other Accession | Q8R1P4, NP_001124500.1 |
| Reactivity | Human |
| Predicted | Mouse |
| Concentration | 0.25 mg/ml |
| Isotype | Rabbit Ig |
| Calculated MW | 25279 Da |
APG10L Antibody (C-term S116) - Additional info | |
| Gene ID 83734 | |
| Other Names ATG10; Ubiquitin-like-conjugating enzyme ATG10; Autophagy-related protein 10 | |
| Target/Specificity This APG10L antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 95-121 amino acids from the C-terminal region of human APG10L. | |
| Dilution WB~~1:100~500WB~~1:1000 IHC~~1:10~50 | |
| Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. | |
| Storage Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. | |
| Precautions APG10L Antibody (C-term S116) is for research use only and not for use in diagnostic or therapeutic procedures. | |
APG10L Antibody (C-term S116) - Protein Information | |
| Name ATG10 | |
| Function E2-like enzyme involved in autophagy. Acts as an E2-like enzyme that catalyzes the conjugation of ATG12 to ATG5. ATG12 conjugation to ATG5 is required for autophagy. Likely serves as an ATG5-recognition molecule. Not involved in ATG12 conjugation to ATG3 (By similarity) | |
| Cellular Location Cytoplasm (Probable). | |
APG10L Antibody (C-term S116) - Related products
AP14315c: APG10L Antibody (C-term S116)
RI10450: ATG10 predesign siRNA
APG10L Antibody (C-term S116) - Application data
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APG10L Antibody (C-term S116) (Cat. #AP14315c) western blot analysis in NCI-H292 cell line lysates (35ug/lane).This demonstrates the APG10L antibody detected the APG10L protein (arrow).
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Western blot analysis of APG10L (arrow) using rabbit polyclonal APG10L Antibody (C-term S116) (Cat. #AP14315c). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the APG10L gene.
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APG10L Antibody (C-term S116) (Cat. #AP14315c)immunohistochemistry analysis in formalin fixed and paraffin embedded human rectum tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of ANKS1B APG10L Antibody (C-term S116) for immunohistochemistry. Clinical relevance has not been evaluated.
APG10L Antibody (C-term S116) - Research Areas
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BACKGROUND
Autophagy is a process for the bulk degradation of cytosolic compartments by lysosomes. ATG10 is an E2-like enzyme involved in 2 ubiquitin-like modifications essential for autophagosome formation: ATG12 (MIM 609608)-ATG5 (MIM 604261) conjugation and modification of a soluble form of MAP-LC3 (MAP1LC3A; MIM 601242), a homolog of yeast Apg8, to a membrane-bound form (Nemoto et al., 2003 [PubMed 12890687]).
REFERENCES
Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010) : Criollo, A., et al. Cell Death Differ. 14(5):1029-1039(2007) Shao, Y., et al. Autophagy 3(1):10-16(2007) Ewing, R.M., et al. Mol. Syst. Biol. 3, 89 (2007) : Boya, P., et al. Mol. Cell. Biol. 25(3):1025-1040(2005)
