|Application ||WB, IF, E|
|Other Accession||P63140, P63139, P25207, Q32KW0, NP_006157.1, Q6RG77|
|Predicted||Bovine, Chicken, Mouse, Rat|
|Calculated MW||22831 Da|
|Antigen Region||17-46 aa|
|Other Names||Nuclear transcription factor Y subunit beta, CAAT box DNA-binding protein subunit B, Nuclear transcription factor Y subunit B, NF-YB, NFYB, HAP3|
|Target/Specificity||This NFYB antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 17-46 amino acids from the N-terminal region of human NFYB.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||NFYB Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Component of the sequence-specific heterotrimeric transcription factor (NF-Y) which specifically recognizes a 5'- CCAAT-3' box motif found in the promoters of its target genes. NF- Y can function as both an activator and a repressor, depending on its interacting cofactors.|
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Provided below are standard protocols that you may find useful for product applications.
The protein encoded by this gene is one subunit of a trimeric complex, forming a highly conserved transcription factor that binds with high specificity to CCAAT motifs in the promoter regions in a variety of genes. This gene product, subunit B, forms a tight dimer with the C subunit, a prerequisite for subunit A association. The resulting trimer binds to DNA with high specificity and affinity. Subunits B and C each contain a histone-like motif. Observation of the histone nature of these subunits is supported by two types of evidence; protein sequence alignments and experiments with mutants.
Stros, M., et al. Nucleic Acids Res. 37(7):2070-2086(2009)
Kang, J.H., et al. J. Cell. Biochem. 104(4):1192-1203(2008)
Raju, C.S., et al. Mol. Biol. Cell 19(7):3008-3019(2008)
Di Agostino, S., et al. Cancer Cell 10(3):191-202(2006)
Kahle, J., et al. Mol. Cell. Biol. 25(13):5339-5354(2005)
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