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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   NFYB Antibody (N-term)   

NFYB Antibody (N-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

  • WB - NFYB Antibody (N-term) AP14334A
    NFYB Antibody (N-term) (Cat. #AP14334a) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the NFYB antibody detected the NFYB protein (arrow).
  • IF - NFYB Antibody (N-term) AP14334A
    Fluorescent confocal image of Hela cell stained with NFYB Antibody (N-term)(Cat#AP14334a). Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with NFYB primary antibody (1:25, 1 h at 37℃). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37℃).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37℃). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min). NFYB immunoreactivity is localized to nucleus significantly.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P25208
Other Accession P63140, P63139, P25207, Q32KW0, NP_006157.1, Q6RG77
Reactivity Human
Predicted Bovine, Chicken, Mouse, Rat
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 22831 Da
Antigen Region 17-46 aa
Additional Information
Gene ID 4801
Other Names Nuclear transcription factor Y subunit beta, CAAT box DNA-binding protein subunit B, Nuclear transcription factor Y subunit B, NF-YB, NFYB, HAP3
Target/Specificity This NFYB antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 17-46 amino acids from the N-terminal region of human NFYB.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsNFYB Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Synonyms HAP3
Function Component of the sequence-specific heterotrimeric transcription factor (NF-Y) which specifically recognizes a 5'- CCAAT-3' box motif found in the promoters of its target genes. NF- Y can function as both an activator and a repressor, depending on its interacting cofactors.
Cellular Location Nucleus.
Research Areas
Citations (0)

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The protein encoded by this gene is one subunit of a trimeric complex, forming a highly conserved transcription factor that binds with high specificity to CCAAT motifs in the promoter regions in a variety of genes. This gene product, subunit B, forms a tight dimer with the C subunit, a prerequisite for subunit A association. The resulting trimer binds to DNA with high specificity and affinity. Subunits B and C each contain a histone-like motif. Observation of the histone nature of these subunits is supported by two types of evidence; protein sequence alignments and experiments with mutants.


Stros, M., et al. Nucleic Acids Res. 37(7):2070-2086(2009)
Kang, J.H., et al. J. Cell. Biochem. 104(4):1192-1203(2008)
Raju, C.S., et al. Mol. Biol. Cell 19(7):3008-3019(2008)
Di Agostino, S., et al. Cancer Cell 10(3):191-202(2006)
Kahle, J., et al. Mol. Cell. Biol. 25(13):5339-5354(2005)

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$ 295.00
$ 99.00
Cat# AP14334A
(40 western blots)
Availability: In Stock
Bulk Size
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