|Application ||WB, E|
|Other Accession||Q6NZQ8, NP_060393.1, NP_001159845.1|
|Calculated MW||32308 Da|
|Antigen Region||163-191 aa|
|Other Names||E3 ubiquitin-protein ligase MARCH1, 632-, Membrane-associated RING finger protein 1, Membrane-associated RING-CH protein I, MARCH-I, RING finger protein 171, MARCH1, RNF171|
|Target/Specificity||This MARCH1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 163-191 amino acids from the Central region of human MARCH1.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||MARCH1 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||E3 ubiquitin-protein ligase that mediates ubiquitination of TFRC, CD86, FAS and MHC class II proteins, such as HLA-DR alpha and beta, and promotes their subsequent endocytosis and sorting to lysosomes via multivesicular bodies. By constitutively ubiquitinating MHC class II proteins in immature dendritic cells, down-regulates their cell surface localization thus sequestering them in the intracellular endosomal system.|
|Cellular Location||Golgi apparatus, trans-Golgi network membrane; Multi-pass membrane protein Lysosome membrane; Multi-pass membrane protein. Cytoplasmic vesicle membrane; Multi-pass membrane protein. Late endosome membrane; Multi-pass membrane protein Early endosome membrane; Multi-pass membrane protein. Cell membrane; Multi-pass membrane protein|
|Tissue Location||Expressed in antigen presenting cells, APCs, located in lymph nodes and spleen. Also expressed in lung Expression is high in follicular B-cells, moderate in dendritic cells and low in splenic T-cells.|
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Provided below are standard protocols that you may find useful for product applications.
MARCH1 is a member of the MARCH family of membrane-bound E3 ubiquitin ligases (EC 188.8.131.52). MARCH proteins add ubiquitin (see MIM 191339) to target lysines in substrate proteins, thereby signaling their vesicular transport between membrane compartments. MARCH1 downregulates the surface expression of major histocompatibility complex (MHC) class II molecules (see MIM 142880) and other glycoproteins by directing them to the late endosomal/lysosomal compartment (Bartee et al., 2004 [PubMed 14722266]; Thibodeau et al., 2008 [PubMed 18389477]; De Gassart et al., 2008 [PubMed 18305173]).
Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010) :
Thibodeau, J., et al. Eur. J. Immunol. 38(5):1225-1230(2008)
De Gassart, A., et al. Proc. Natl. Acad. Sci. U.S.A. 105(9):3491-3496(2008)
Bartee, E., et al. J. Virol. 78(3):1109-1120(2004)
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