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>   home   >   Products   >   Primary Antibodies   >   Immunology   >   MARCH1 Antibody (Center)   

MARCH1 Antibody (Center)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

  • WB - MARCH1 Antibody (Center) AP14345c
    MARCH1 Antibody (Center) (Cat. #AP14345c) western blot analysis in mouse heart tissue lysates (35ug/lane).This demonstrates the MARCH1 antibody detected the MARCH1 protein (arrow).
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q8TCQ1
Other Accession Q6NZQ8, NP_060393.1, NP_001159845.1
Reactivity Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 32308 Da
Antigen Region 163-191 aa
Additional Information
Gene ID 55016
Other Names E3 ubiquitin-protein ligase MARCH1, 632-, Membrane-associated RING finger protein 1, Membrane-associated RING-CH protein I, MARCH-I, RING finger protein 171, MARCH1, RNF171
Target/Specificity This MARCH1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 163-191 amino acids from the Central region of human MARCH1.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsMARCH1 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Synonyms RNF171
Function E3 ubiquitin-protein ligase that mediates ubiquitination of TFRC, CD86, FAS and MHC class II proteins, such as HLA-DR alpha and beta, and promotes their subsequent endocytosis and sorting to lysosomes via multivesicular bodies. By constitutively ubiquitinating MHC class II proteins in immature dendritic cells, down-regulates their cell surface localization thus sequestering them in the intracellular endosomal system.
Cellular Location Golgi apparatus, trans-Golgi network membrane; Multi-pass membrane protein Lysosome membrane; Multi-pass membrane protein. Cytoplasmic vesicle membrane; Multi-pass membrane protein. Late endosome membrane; Multi-pass membrane protein Early endosome membrane; Multi-pass membrane protein. Cell membrane; Multi-pass membrane protein
Tissue Location Expressed in antigen presenting cells, APCs, located in lymph nodes and spleen. Also expressed in lung Expression is high in follicular B-cells, moderate in dendritic cells and low in splenic T-cells.
Research Areas
Citations (0)

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MARCH1 is a member of the MARCH family of membrane-bound E3 ubiquitin ligases (EC MARCH proteins add ubiquitin (see MIM 191339) to target lysines in substrate proteins, thereby signaling their vesicular transport between membrane compartments. MARCH1 downregulates the surface expression of major histocompatibility complex (MHC) class II molecules (see MIM 142880) and other glycoproteins by directing them to the late endosomal/lysosomal compartment (Bartee et al., 2004 [PubMed 14722266]; Thibodeau et al., 2008 [PubMed 18389477]; De Gassart et al., 2008 [PubMed 18305173]).


Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010) :
Thibodeau, J., et al. Eur. J. Immunol. 38(5):1225-1230(2008)
De Gassart, A., et al. Proc. Natl. Acad. Sci. U.S.A. 105(9):3491-3496(2008)
Bartee, E., et al. J. Virol. 78(3):1109-1120(2004)

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$ 295.00
$ 99.00
Cat# AP14345c
(40 western blots)
Availability: In Stock
Bulk Size
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