|Application ||WB, E|
|Other Accession||Q8R4I4, NP_006863.2|
|Calculated MW||52444 Da|
|Antigen Region||399-428 aa|
|Other Names||TFIIA-alpha and beta-like factor, General transcription factor II A, 1-like factor, GTF2A1L, ALF, GTF2A1LF|
|Target/Specificity||This GTF2A1L antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 399-428 amino acids from the C-terminal region of human GTF2A1L.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||GTF2A1L Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||May function as a testis specific transcription factor. Binds DNA in conjunction with GTF2A2 and TBP (the TATA-binding protein) and together with GTF2A2, allows mRNA transcription.|
|Cellular Location||Nucleus. Note=Mainly localizes in the annulus and partly in acrosomal cap area of spermatozoa|
|Tissue Location||Testis specific. Detected in adult testis mostly in round and elongating spermatids (at protein level) Detected in testis.|
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Provided below are standard protocols that you may find useful for product applications.
The assembly and stability of the RNA polymerase II transcription pre-initiation complex on a eukaryotic core promoter involve the effects of TFIIA on the interaction between TATA-binding protein (TBP) and DNA. This gene encodes a germ cell-specific counterpart of the large (alpha/beta) subunit of general transcription factor TFIIA that is able to stabilize the binding of TBP to DNA and may be uniquely important to testis biology. Alternative splicing for this locus has been observed and two variants, encoding distinct isoforms, have been identified. Co-transcription of this gene and the neighboring upstream gene generates a rare transcript (SALF), which encodes a fusion protein comprised of sequence sharing identity with each individual gene product.
Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010) :
Kim, M., et al. J. Biol. Chem. 281(45):34288-34298(2006)
Howe, M.L., et al. Stem Cells Dev. 15(2):175-190(2006)
Huang, M., et al. Int. J. Mol. Med. 17(4):599-604(2006)
Upadhyaya, A.B., et al. J. Biol. Chem. 277(37):34208-34216(2002)
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