|Application ||WB, IF, E|
|Other Accession||Q9JMD0, NP_001027464.1, NP_001091977.1|
|Calculated MW||50751 Da|
|Antigen Region||88-116 aa|
|Other Names||BUB3-interacting and GLEBS motif-containing protein ZNF207, BuGZ, Zinc finger protein 207, ZNF207, BUGZ|
|Target/Specificity||This ZNF207 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 88-116 amino acids from the N-terminal region of human ZNF207.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||ZNF207 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Kinetochore- and microtubule-binding protein that plays a key role in spindle assembly (PubMed:24462186, PubMed:24462187, PubMed:26388440). ZNF207/BuGZ is mainly composed of disordered low-complexity regions and undergoes phase transition or coacervation to form temperature-dependent liquid droplets. Coacervation promotes microtubule bundling and concentrates tubulin, promoting microtubule polymerization and assembly of spindle and spindle matrix by concentrating its building blocks (PubMed:26388440). Also acts as a regulator of mitotic chromosome alignment by mediating the stability and kinetochore loading of BUB3 (PubMed:24462186, PubMed:24462187). Mechanisms by which BUB3 is protected are unclear: according to a first report, ZNF207/BuGZ may act by blocking ubiquitination and proteasomal degradation of BUB3 (PubMed:24462186). According to another report, the stabilization is independent of the proteasome (PubMed:24462187).|
|Cellular Location||Nucleus. Chromosome, centromere, kinetochore Cytoplasm, cytoskeleton, spindle Note=Localizes primarily to the nucleus in interphase, concentrates at kinetochores prior to nuclear envelope breakdown and during early prometaphase, and disappears from kinetochores upon microtubule-binding.|
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Provided below are standard protocols that you may find useful for product applications.
ZNF207 is a new candidate transcription factor.
Andersen, J.S., et al. Nature 433(7021):77-83(2005)
Pahl, P.M., et al. Genomics 53(3):410-412(1998)
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