|Application ||WB, E|
|Other Accession||Q6A028, P0C1G6|
|Calculated MW||68998 Da|
|Antigen Region||227-256 aa|
|Other Names||Switch-associated protein 70, SWAP-70, SWAP70, KIAA0640|
|Target/Specificity||This SWAP70 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 227-256 amino acids from the Central region of human SWAP70.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||SWAP70 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Phosphatidylinositol 3,4,5-trisphosphate-dependent guanine nucleotide exchange factor (GEF) which, independently of RAS, transduces signals from tyrosine kinase receptors to RAC. It also mediates signaling of membrane ruffling. Regulates the actin cytoskeleton as an effector or adapter protein in response to agonist stimulated phosphatidylinositol (3,4)-bisphosphate production and cell protrusion (By similarity).|
|Cellular Location||Cytoplasm. Cell membrane. Nucleus. Cell projection, lamellipodium Cytoplasm, cytoskeleton. Note=In resting B-cells it is localized mainly in the cytoplasm and upon cell activation it is recruited to the plasma membrane and then translocates to the nucleus (PubMed:10681448). In activated, class-switching B-cells it is associated with membrane IgG but not IgM (PubMed:10681448). Localized to loose actin filament arrays located behind actively extending lamellipodia (PubMed:12925760)|
|Tissue Location||Expressed only in mature B-cells including those associated with mucosa-associated tissue and bronchus- associated tissue (PubMed:10681448). Widely expressed. Abundant in spleen, and fairly abundant in kidney, lung and liver. Also found in monocytes and macrophages (PubMed:12925760)|
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Provided below are standard protocols that you may find useful for product applications.
SWAP70 is a phosphatidylinositol 3,4,5-trisphosphate-dependent guanine nucleotide exchange factor (GEF) which, independently of RAS, transduces signals from tyrosine kinase receptors to RAC. It also mediates signaling of membrane ruffling. This protein regulates the actin cytoskeleton as an effector or adapter protein in response to agonist stimulated phosphatidylinositol (3,4)-bisphosphate production and cell protrusion.
Hilpel, et al., Mol. Biol. Cell 14(8):3242-3253 (2003).
Rapalus, L., et al., Clin. Immunol. 101(3):270-275 (2001).
Masat, L., et al., Proc. Natl. Acad. Sci. U.S.A. 97(5):2180-2184 (2000).
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