|Application ||IF, WB, IHC-P, E|
|Calculated MW||33295 Da|
|Antigen Region||29-58 aa|
|Other Names||Histamine N-methyltransferase, HMT, HNMT|
|Target/Specificity||This HNMT antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 29-58 amino acids from the N-terminal region of human HNMT.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||HNMT Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Inactivates histamine by N-methylation. Plays an important role in degrading histamine and in regulating the airway response to histamine.|
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Provided below are standard protocols that you may find useful for product applications.
In mammals, histamine is metabolized by two major pathways: N(tau)-methylation via histamine N-methyltransferase and oxidative deamination via diamine oxidase. HNMT is the first enzyme which is found in the cytosol and uses S-adenosyl-L-methionine as the methyl donor. In the mammalian brain, the neurotransmitter activity of histamine is controlled by N(tau)-methylation as diamine oxidase is not found in the central nervous system. A common genetic polymorphism affects the activity levels of this gene product in red blood cells.
Aksoy, S., et al., Biochem. Biophys. Res. Commun. 219(2):548-554 (1996).
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