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AKAP4 Antibody (N-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

  • WB - AKAP4 Antibody (N-term) AP14462a
    AKAP4 Antibody (N-term) (Cat. #AP14462a) western blot analysis in HL-60 cell line lysates (35ug/lane).This demonstrates the AKAP4 antibody detected the AKAP4 protein (arrow).
  • IHC-P - AKAP4 Antibody (N-term) AP14462a
    AKAP4 Antibody (N-term) (AP14462a)immunohistochemistry analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of AKAP4 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q5JQC9
Other Accession NP_003877.2, NP_647450.1
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 94477 Da
Antigen Region 10-36 aa
Additional Information
Gene ID 8852
Other Names A-kinase anchor protein 4, AKAP-4, A-kinase anchor protein 82 kDa, AKAP 82, hAKAP82, Major sperm fibrous sheath protein, HI, Protein kinase A-anchoring protein 4, PRKA4, AKAP4 (HGNC:374), AKAP82
Target/Specificity This AKAP4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 10-36 amino acids from the N-terminal region of human AKAP4.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsAKAP4 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name AKAP4 (HGNC:374)
Synonyms AKAP82
Function Major structural component of sperm fibrous sheath. Plays a role in sperm motility.
Cellular Location Cell projection, cilium, flagellum. Note=Localizes to the principle piece of the sperm flagellum
Tissue Location Testis specific; only expressed in round spermatids.
Research Areas
Citations (0)

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The A-kinase anchor proteins (AKAPs) are a group of structurally diverse proteins, which have the common function of binding to the regulatory subunit of protein kinase A (PKA) and confining the holoenzyme to discrete locations within the cell. This gene encodes a member of the AKAP family. The encoded protein is localized to the sperm flagellum and may be involved in the regulation of sperm motility. Alternative splicing of this gene results in two transcript variants encoding different isoforms.


Moretti, E., et al. Asian J. Androl. 9(5):641-649(2007)
Lefievre, L., et al. Proteomics 7(17):3066-3084(2007)
Ross, M.T., et al. Nature 434(7031):325-337(2005)
Brown, P.R., et al. Biol. Reprod. 68(6):2241-2248(2003)
Ficarro, S., et al. J. Biol. Chem. 278(13):11579-11589(2003)

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Cat# AP14462a
(40 western blots)
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