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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   SPRED1 Antibody (C-term)   

SPRED1 Antibody (C-term)

Purified Rabbit Polyclonal Antibody (Pab)

  • WB - SPRED1 Antibody (C-term) AP1449b
    Western blot analysis of SPRED1 Antibody (C-term) polyclonal antibody(Cat.#AP1449b)(arrow). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the SPRED1 gene (Lane 2) (Origene Technologies).
  • IHC-P - SPRED1 Antibody (C-term) AP1449b
    Formalin-fixed and paraffin-embedded human brain tissue reacted with SPRED1 antibody (C-term) (Cat.#AP1449b), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q7Z699
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 50477 Da
Antigen Region 302-331 aa
Additional Information
Gene ID 161742
Other Names Sprouty-related, EVH1 domain-containing protein 1, Spred-1, hSpred1, SPRED1
Target/Specificity This SPRED1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 302-331 amino acids from the C-terminal region of human SPRED1.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsSPRED1 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Function Tyrosine kinase substrate that inhibits growth-factor- mediated activation of MAP kinase. Negatively regulates hematopoiesis of bone marrow (By similarity).
Cellular Location Cell membrane; Peripheral membrane protein Membrane, caveola; Peripheral membrane protein. Nucleus. Note=Localized in cholesterol-rich membrane raft/caveola fractions
Tissue Location Weakly expressed in embryonic cell line HEK293.
Research Areas
Citations (0)

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SPRED1 is a tyrosine kinase substrate that inhibits growth-factor-mediated activation of MAP kinase.


Brems,H., Nat. Genet. 39 (9), 1120-1126 (2007)
Szafranski,K., Genome Biol. 8 (8), R154 (2007)
Yoshida,T., Oncogene 25 (45), 6056-6066 (2006)
King,J.A., Biochem. J. 388 (PT 2), 445-454 (2005)

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$ 295.00
$ 99.00
Cat# AP1449b
(40 western blots)
Availability: In Stock
Bulk Size
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