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PYGM Antibody (C-term)

Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - PYGM Antibody (C-term) AP1450b
    Western blot analysis of PYGM (arrow) using rabbit polyclonal PYGM Antibody (C-term) (Cat.#AP1450b). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PYGM gene (Lane 2) (Origene Technologies).
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  • WB - PYGM Antibody (C-term) AP1450b
    Anti-PYGM Antibody (C-term) at 1:8000 dilution + human skeletal muscle lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 97 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
    detail
  • IHC-P - PYGM Antibody (C-term) AP1450b
    Formalin-fixed and paraffin-embedded human skeletal muscle tissue reacted with PYGM antibody (C-term) (Cat.#AP1450b), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
    detail
  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, E
Primary Accession P11217
Other Accession Q8HXW4
Reactivity Human
Predicted Monkey
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 97092 Da
Antigen Region 698-727 aa
Additional Information
Gene ID 5837
Other Names Glycogen phosphorylase, muscle form, Myophosphorylase, PYGM
Target/Specificity This PYGM antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 698-727 amino acids from the C-terminal region of human PYGM.
Dilution WB~~1:8000
IHC-P~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsPYGM Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name PYGM (HGNC:9726)
Function Allosteric enzyme that catalyzes the rate-limiting step in glycogen catabolism, the phosphorolytic cleavage of glycogen to produce glucose-1-phosphate, and plays a central role in maintaining cellular and organismal glucose homeostasis.
Research Areas
Citations (0)
citation

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Background

PYGM catalyzes and regulates the breakdown of glycogen to glucose-1-phosphate. Defects in PYGM are the cause of glycogen storage disease type 5 (GSD5), also known as McArdle disease. GSD5 is a metabolic disorder resulting in myopathy characterized by exercise intolerance, cramps, muscle weakness and recurrent myoglobinuria.

References

Tsoi, S.C., et al., J. Soc. Gynecol. Investig. 10(8):496-502 (2003).
Bruno, C., et al., Neuromuscul. Disord. 12(5):498-500 (2002).
Hadjigeorgiou, G.M., et al., Neuromuscul. Disord. 12(9):824-827 (2002).
Deschauer, M., et al., Mol. Genet. Metab. 74(4):489-491 (2001).
Kubisch, C., et al., Hum. Mutat. 12(1):27-32 (1998).

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$ 365.00
$ 140.00
Cat# AP1450b
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