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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   PYGM Antibody (C-term)   

PYGM Antibody (C-term)

Purified Rabbit Polyclonal Antibody (Pab)

  • WB - PYGM Antibody (C-term) AP1450b
    Anti-PYGM Antibody (C-term) at 1:8000 dilution + human skeletal muscle lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 97 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • WB - PYGM Antibody (C-term) AP1450b
    Western blot analysis of PYGM (arrow) using rabbit polyclonal PYGM Antibody (C-term) (Cat.#AP1450b). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PYGM gene (Lane 2) (Origene Technologies).
  • IHC-P - PYGM Antibody (C-term) AP1450b
    Formalin-fixed and paraffin-embedded human skeletal muscle tissue reacted with PYGM antibody (C-term) (Cat.#AP1450b), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P11217
Other Accession Q8HXW4
Reactivity Human
Predicted Monkey
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 97092 Da
Antigen Region 698-727 aa
Additional Information
Gene ID 5837
Other Names Glycogen phosphorylase, muscle form, Myophosphorylase, PYGM
Target/Specificity This PYGM antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 698-727 amino acids from the C-terminal region of human PYGM.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsPYGM Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Function Phosphorylase is an important allosteric enzyme in carbohydrate metabolism. Enzymes from different sources differ in their regulatory mechanisms and in their natural substrates. However, all known phosphorylases share catalytic and structural properties.
Research Areas
Citations (0)

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PYGM catalyzes and regulates the breakdown of glycogen to glucose-1-phosphate. Defects in PYGM are the cause of glycogen storage disease type 5 (GSD5), also known as McArdle disease. GSD5 is a metabolic disorder resulting in myopathy characterized by exercise intolerance, cramps, muscle weakness and recurrent myoglobinuria.


Tsoi, S.C., et al., J. Soc. Gynecol. Investig. 10(8):496-502 (2003).
Bruno, C., et al., Neuromuscul. Disord. 12(5):498-500 (2002).
Hadjigeorgiou, G.M., et al., Neuromuscul. Disord. 12(9):824-827 (2002).
Deschauer, M., et al., Mol. Genet. Metab. 74(4):489-491 (2001).
Kubisch, C., et al., Hum. Mutat. 12(1):27-32 (1998).

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$ 295.00
$ 99.00
Cat# AP1450b
(40 western blots)
Availability: In Stock
Bulk Size
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