|Application ||WB, IHC-P, E|
|Other Accession||P49717, P30664, NP_005905.2, NP_877423.1|
|Calculated MW||96558 Da|
|Antigen Region||637-665 aa|
|Other Names||DNA replication licensing factor MCM4, CDC21 homolog, P1-CDC21, MCM4, CDC21|
|Target/Specificity||This MCM4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 637-665 amino acids from the C-terminal region of human MCM4.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||MCM4 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity.|
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Provided below are standard protocols that you may find useful for product applications.
The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are essential for the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. The MCM complex consisting of this protein and MCM2, 6 and 7 proteins possesses DNA helicase activity, and may act as a DNA unwinding enzyme. The phosphorylation of this protein by CDC2 kinase reduces the DNA helicase activity and chromatin binding of the MCM complex. This gene is mapped to a region on the chromosome 8 head-to-head next to the PRKDC/DNA-PK, a DNA-activated protein kinase involved in the repair of DNA double-strand breaks. Alternatively spliced transcript variants encoding the same protein have been reported.
Olson, J.E., et al. Breast Cancer Res. Treat. (2010) In press :
Qian, Z., et al. PLoS Pathog. 6 (3), E1000814 (2010) :
Ladstein, R.G., et al. BMC Cancer 10, 140 (2010) :
Hosgood, H.D. III, et al. Occup Environ Med 66(12):848-853(2009)
Enjuanes, A., et al. Cancer Res. 68(24):10178-10186(2008)
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