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>   home   >   Products   >   Primary Antibodies   >   MCM4 Antibody (C-term)   

MCM4 Antibody (C-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

  • WB - MCM4 Antibody (C-term) AP14534B
    MCM4 Antibody (C-term) (Cat. #AP14534b) western blot analysis in CEM cell line lysates (35ug/lane).This demonstrates the MCM4 antibody detected the MCM4 protein (arrow).
  • IHC-P - MCM4 Antibody (C-term) AP14534B
    MCM4 Antibody (C-term) (AP14534b)immunohistochemistry analysis in formalin fixed and paraffin embedded human tonsil tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of MCM4 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P33991
Other Accession P49717, P30664, NP_005905.2, NP_877423.1
Reactivity Human
Predicted Xenopus, Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 96558 Da
Antigen Region 637-665 aa
Additional Information
Gene ID 4173
Other Names DNA replication licensing factor MCM4, CDC21 homolog, P1-CDC21, MCM4, CDC21
Target/Specificity This MCM4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 637-665 amino acids from the C-terminal region of human MCM4.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsMCM4 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name MCM4
Synonyms CDC21
Function Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity.
Cellular Location Nucleus.
Citations (0)

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The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are essential for the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. The MCM complex consisting of this protein and MCM2, 6 and 7 proteins possesses DNA helicase activity, and may act as a DNA unwinding enzyme. The phosphorylation of this protein by CDC2 kinase reduces the DNA helicase activity and chromatin binding of the MCM complex. This gene is mapped to a region on the chromosome 8 head-to-head next to the PRKDC/DNA-PK, a DNA-activated protein kinase involved in the repair of DNA double-strand breaks. Alternatively spliced transcript variants encoding the same protein have been reported.


Olson, J.E., et al. Breast Cancer Res. Treat. (2010) In press :
Qian, Z., et al. PLoS Pathog. 6 (3), E1000814 (2010) :
Ladstein, R.G., et al. BMC Cancer 10, 140 (2010) :
Hosgood, H.D. III, et al. Occup Environ Med 66(12):848-853(2009)
Enjuanes, A., et al. Cancer Res. 68(24):10178-10186(2008)

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$ 295.00
$ 99.00
Cat# AP14534B
(40 western blots)
Availability: In Stock
Bulk Size
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