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>   home   >   Products   >   Primary Antibodies   >   Cell Biology   >   TPX2 Antibody (Center)   

TPX2 Antibody (Center)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - TPX2 Antibody (Center) AP14553c
    TPX2 Antibody (Center) (Cat. #AP14553c) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the TPX2 antibody detected the TPX2 protein (arrow).
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, E
Primary Accession Q9ULW0
Other Accession A6H6Z7, NP_036244.2
Reactivity Human
Predicted Bovine
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 85653 Da
Antigen Region 419-448 aa
Additional Information
Gene ID 22974
Other Names Targeting protein for Xklp2, Differentially expressed in cancerous and non-cancerous lung cells 2, DIL-2, Hepatocellular carcinoma-associated antigen 519, Hepatocellular carcinoma-associated antigen 90, Protein fls353, Restricted expression proliferation-associated protein 100, p100, TPX2, C20orf1, C20orf2, DIL2, HCA519
Target/Specificity This TPX2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 419-448 amino acids from the Central region of human TPX2.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsTPX2 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name TPX2
Synonyms C20orf1, C20orf2, DIL2, HCA519
Function Spindle assembly factor required for normal assembly of mitotic spindles. Required for normal assembly of microtubules during apoptosis. Required for chromatin and/or kinetochore dependent microtubule nucleation. Mediates AURKA localization to spindle microtubules (PubMed:18663142, PubMed:19208764). Activates AURKA by promoting its autophosphorylation at 'Thr-288' and protects this residue against dephosphorylation (PubMed:18663142, PubMed:19208764). TPX2 is inactivated upon binding to importin- alpha (PubMed:26165940). At the onset of mitosis, GOLGA2 interacts with importin-alpha, liberating TPX2 from importin-alpha, allowing TPX2 to activates AURKA kinase and stimulates local microtubule nucleation (PubMed:26165940).
Cellular Location Nucleus. Cytoplasm, cytoskeleton, spindle. Cytoplasm, cytoskeleton, spindle pole Note=During mitosis it is strictly associated with the spindle pole and with the mitotic spindle, whereas during S and G2, it is diffusely distributed throughout the nucleus. Is released from the nucleus in apoptotic cells and is detected on apoptotic microtubules.
Tissue Location Expressed in lung carcinoma cell lines but not in normal lung tissues
Research Areas
Citations (0)

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Background

TPX2 is a spindle assembly factor. Required for normal assembly of mitotic spindles. Required for normal assembly of microtubules during apoptosis. Required for chromatin and/or kinetochore dependent microtubule nucleation. Mediates AURKA localization to spindle microtubules. Activates AURKA by promoting its autophosphorylation at 'Thr-288' and protects this residue against dephosphorylation.

References

Olson, J.E., et al. Breast Cancer Res. Treat. (2010) In press :
Hosgood, H.D. III, et al. Occup Environ Med 66(12):848-853(2009)
Bibby, R.A., et al. J. Biol. Chem. 284(48):33177-33184(2009)
Shigeishi, H., et al. Int. J. Oncol. 34(6):1565-1571(2009)
Moss, D.K., et al. J. Cell. Sci. 122 (PT 5), 644-655 (2009) :

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$ 295.00
$ 99.00
Cat# AP14553c
Size:
Quantity:
(40 western blots)
Availability: In Stock
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