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IDE Antibody (Center)

Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - IDE Antibody (Center) AP1455c
    All lanes : Anti-IDE Antibody (Center) at 1:2000 dilution Lane 1: Hela whole cell lysate Lane 2: HepG2 whole cell lysate Lane 3: A375 whole cell lysate Lane 4: K562 whole cell lysate Lane 5: mouse liver lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 118 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • WB - IDE Antibody (Center) AP1455c
    All lanes : Anti-IDE Antibody (Center) at 1:2000 dilution Lane 1: A375 whole cell lysate Lane 2: Hela whole cell lysate Lane 3: K562 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 118 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • WB - IDE Antibody (Center) AP1455c
    Western blot analysis of anti-IDE Antibody (Center) (Cat.#AP1455c) in A375 cell line lysates (35ug/lane). IDE (arrow) was detected using the purified Pab.
  • IHC-P - IDE Antibody (Center) AP1455c
    Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with IDE antibody (Center)(Cat.#AP1455c), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
  • WB - IDE Antibody (Center) AP1455c
    Western blot analysis of lysate from K562 cell line, using IDE Antibody (Center)(Cat. #AP1455c). AP1455c was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug.
  • SPECIFICATION
  • CITATIONS: 2
  • PROTOCOLS
  • BACKGROUND
Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, E
Primary Accession P14735
Other Accession P35559, Q9JHR7, Q24K02
Reactivity Human, Mouse
Predicted Mouse, Rat, Bovine
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 117968 Da
Antigen Region 406-435 aa
Additional Information
Gene ID 3416
Other Names Insulin-degrading enzyme, Abeta-degrading protease, Insulin protease, Insulinase, Insulysin, IDE
Target/Specificity This IDE antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 406-435 amino acids from the Central region of human IDE.
Dilution WB~~1:1000
IHC-P~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsIDE Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name IDE
Function Plays a role in the cellular breakdown of insulin, IAPP, glucagon, bradykinin, kallidin and other peptides, and thereby plays a role in intercellular peptide signaling. Degrades amyloid formed by APP and IAPP. May play a role in the degradation and clearance of naturally secreted amyloid beta-protein by neurons and microglia.
Cellular Location Cytoplasm. Cell membrane. Secreted. Note=Present at the cell surface of neuron cells The membrane-associated isoform is approximately 5 kDa larger than the known cytosolic isoform
Research Areas
Citations ( 0 )

Background

IDE belongs to a protease family responsible for intercellular peptide signalling. Though its role in the cellular processing of insulin has not yet been defined, insulin-degrading enzyme is thought to be involved in the termination of the insulin response.

References

Vepsalainen,S.,J. Med. Genet. 44 (9), 606-608 (2007)
Kim,M.,J. Biol. Chem. 282 (11), 7825-7832 (2007)
Radulescu,R.T.,Int. J. Oncol. 30 (1), 73-80 (2007)
Li,Q.,Cell 127 (2), 305-316 (2006)

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$ 295.00
$ 99.00
Cat# AP1455c
Size:
Quantity:
(40 western blots)
Availability: In Stock
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