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> home > Products > Primary Antibodies > Antibody Collections > Host cell receptor for virus entry > IDE Antibody (Center)
IDE Antibody (Center)
Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS: 2
- PROTOCOLS
- BACKGROUND
Application 
| WB, IHC-P, E |
|---|---|
| Primary Accession | P14735 |
| Other Accession | P35559, Q9JHR7, Q24K02 |
| Reactivity | Human, Mouse |
| Predicted | Bovine, Rat |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Calculated MW | 117968 Da |
| Antigen Region | 406-435 aa |
| Gene ID | 3416 |
|---|---|
| Other Names | Insulin-degrading enzyme, Abeta-degrading protease, Insulin protease, Insulinase, Insulysin, IDE |
| Target/Specificity | This IDE antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 406-435 amino acids from the Central region of human IDE. |
| Dilution | WB~~1:2000 IHC-P~~1:10~50 |
| Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | IDE Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | IDE {ECO:0000303|PubMed:20364150, ECO:0000312|HGNC:HGNC:5381} |
|---|---|
| Function | Plays a role in the cellular breakdown of insulin, APP peptides, IAPP peptides, natriuretic peptides, glucagon, bradykinin, kallidin, and other peptides, and thereby plays a role in intercellular peptide signaling (PubMed:2293021, PubMed:10684867, PubMed:26968463, PubMed:17051221, PubMed:17613531, PubMed:18986166, PubMed:19321446, PubMed:23922390, PubMed:24847884, PubMed:26394692, PubMed:29596046, PubMed:21098034). Substrate binding induces important conformation changes, making it possible to bind and degrade larger substrates, such as insulin (PubMed:23922390, PubMed:26394692, PubMed:29596046). Contributes to the regulation of peptide hormone signaling cascades and regulation of blood glucose homeostasis via its role in the degradation of insulin, glucagon and IAPP (By similarity). Plays a role in the degradation and clearance of APP-derived amyloidogenic peptides that are secreted by neurons and microglia (PubMed:9830016, PubMed:26394692) (Probable). Degrades the natriuretic peptides ANP, BNP and CNP, inactivating their ability to raise intracellular cGMP (PubMed:21098034). Also degrades an aberrant frameshifted 40-residue form of NPPA (fsNPPA) which is associated with familial atrial fibrillation in heterozygous patients (PubMed:21098034). Involved in antigen processing. Produces both the N terminus and the C terminus of MAGEA3-derived antigenic peptide (EVDPIGHLY) that is presented to cytotoxic T lymphocytes by MHC class I. |
| Cellular Location | Cytoplasm, cytosol. Cell membrane {ECO:0000250|UniProtKB:P35559}. Secreted Note=Present at the cell surface of neuron cells. The membrane- associated isoform is approximately 5 kDa larger than the known cytosolic isoform |
| Tissue Location | Detected in brain and in cerebrospinal fluid (at protein level). |
Research Areas
Citations ( 0 )
Application Protocols
Provided below are standard protocols that you may find useful for product applications.
Background
IDE belongs to a protease family responsible for intercellular peptide signalling. Though its role in the cellular processing of insulin has not yet been defined, insulin-degrading enzyme is thought to be involved in the termination of the insulin response.
References
Vepsalainen,S.,J. Med. Genet. 44 (9), 606-608 (2007)
Kim,M.,J. Biol. Chem. 282 (11), 7825-7832 (2007)
Radulescu,R.T.,Int. J. Oncol. 30 (1), 73-80 (2007)
Li,Q.,Cell 127 (2), 305-316 (2006)
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$ 182.50
$ 70.00
Cat# AP1455c
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