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Mouse Sgk223 Antibody (N-term)
Affinity Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, E |
|---|---|
| Primary Accession | Q571I4 |
| Other Accession | NP_766499.2 |
| Reactivity | Mouse |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Calculated MW | 147945 Da |
| Antigen Region | 195-224 aa |
| Gene ID | 244418 |
|---|---|
| Other Names | Tyrosine-protein kinase SgK223, Sugen kinase 223, Sgk223, D8Ertd82e |
| Target/Specificity | This Mouse Sgk223 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 195-224 amino acids from the N-terminal region of mouse Sgk223. |
| Dilution | WB~~1:1000 |
| Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | Mouse Sgk223 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | Prag1 {ECO:0000312|MGI:MGI:1196223} |
|---|---|
| Function | Catalytically inactive protein kinase that acts as a scaffold protein (By similarity). Functions as an effector of the small GTPase RND2, which stimulates RhoA activity and inhibits NGF-induced neurite outgrowth (By similarity). Promotes Src family kinase (SFK) signaling by regulating the subcellular localization of CSK, a negative regulator of these kinases, leading to the regulation of cell morphology and motility by a CSK-dependent mechanism (By similarity). Acts as a critical coactivator of Notch signaling (PubMed:25038227). |
| Cellular Location | Cytoplasm {ECO:0000250|UniProtKB:D3ZMK9}. Nucleus. Cell junction, focal adhesion {ECO:0000250|UniProtKB:Q86YV5}. Note=Colocalized with NOTCH1 in the nucleus (PubMed:25038227) |
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Provided below are standard protocols that you may find useful for product applications.
Background
PRAGMIN is also known as SGK223 (sugen kinase 223). The rat homolog was originally identified in a yeast two-hybrid screen for Rnd2 effector proteins expressed in neural cells. Rat Pragmin was found to stimulate RhoA activity for the regulation of neurite outgrowth.
References
Tanaka, H., et al. J. Biol. Chem. 281(15):10355-10364(2006)
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