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ALDH1A1 Antibody (Center)Purified Rabbit Polyclonal Antibody (Pab)
| Country | United States
Ordering Information
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| Catalog # | Size | Availability | Price | |
| AP1465c | 0.1 mg 400 ul | In Stock | $ 255.00 | DISCONTINED INQUIRE CLICK INQUIRE Add to cart |
- Specification
- Citiations : 0
- Reviews
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ALDH1A1 Antibody (Center) - Product info | |
| Application | WB, IHC, IF, FC
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| Primary Accession | P00352 |
| Other Accession | O35945, Q8HYE4 |
| Reactivity | Human |
| Predicted | Mouse, Monkey |
| Concentration | 0.25 mg/ml |
| Isotype | Rabbit Ig |
| Calculated MW | 54862 Da |
ALDH1A1 Antibody (Center) - Additional info | |
| Gene ID 216 | |
| Other Names ALDH1A1; ALDC; ALDH1; PUMB1; Retinal dehydrogenase 1; ALDH-E1; ALHDII; Aldehyde dehydrogenase family 1 member A1; Aldehyde dehydrogenase, cytosolic | |
| Target/Specificity This ALDH1A1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 309~339 amino acids from the center region of human ALDH1A1. | |
| Dilution WB~~1:100~500WB~~1:1000 IHC~~1:10~50 IF~~1:10~50 FC~~1:10~50 | |
| Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. | |
| Storage Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. | |
| Precautions ALDH1A1 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures. | |
ALDH1A1 Antibody (Center) - Protein Information | |
| Name ALDH1A1 | |
| Synonyms ALDC, ALDH1, PUMB1 | |
| Function Binds free retinal and cellular retinol-binding protein- bound retinal. Can convert/oxidize retinaldehyde to retinoic acid (By similarity) | |
| Cellular Location Cytoplasm. | |
ALDH1A1 Antibody (Center) - Related products
AM1846a: ALDH1A1 Antibody (Ascites)
AP1445a: ALDH1A1 Antibody (N-term)
AP1465c: ALDH1A1 Antibody (Center)
RI10225: ALDH1A1 predesign siRNA
LY12320a: AL1A1 Over-expression Lysate
BP1445a: ALDH1A1 Antibody (N-term) Blocking Peptide
BP1465c: ALDH1A1 Antibody (Center) Blocking Peptide
ALDH1A1 Antibody (Center) - Application data
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Western blot analysis of ALDH1A1 (arrow) using rabbit polyclonal ALDH1A1 Antibody (Center) (Cat# AP1465c). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the ALDH1A1 gene (Lane 2) (Origene Technologies).
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Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with ALDH1A1 antibody (Center)(Cat.#AP1465c), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
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Immunofluorescence analysis of anti-ALDH1A1 Antibody (Center) in HeLa cells. 0.025 mg/ml primary antibody was followed by Alexa-Fluor-546-conjugated donkey anti-rabbit lgG (H+L). Alexa-Fluor-546 emits orange fluorescence. Blue counterstaining is DAPI.
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Flow cytometric analysis of HepG2 cells using ALDH1A1 Antibody (Center)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
ALDH1A1 Antibody (Center) - Research Areas
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BACKGROUND
ALDH1A1 belongs to the aldehyde dehydrogenases family of proteins. Aldehyde dehydrogenase is the second enzyme of the major oxidative pathway of alcohol metabolism. Two major liver isoforms of this enzyme, cytosolic and mitochondrial, can be distinguished by their electrophoretic mobilities, kinetic properties, and subcellular localizations. Most Caucasians have two major isozymes, while approximately 50% of Orientals have only the cytosolic isozyme, missing the mitochondrial isozyme. A remarkably higher frequency of acute alcohol intoxication among Orientals than among Caucasians could be related to the absence of the mitochondrial isozyme.
REFERENCES
Moore,S., J Stud Alcohol Drugs 68 (2), 192-196 (2007) Collard,F., Biochimie 89 (3), 369-373 (2007)
