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ALDH1A1 AntibodyPeptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
| Country | United States
Ordering Information
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| Catalog # | Size | Availability | Price | |
| AP1465d | 0.1 mg 400 ul | In Stock | $ 255.00 | DISCONTINED INQUIRE CLICK INQUIRE Add to cart |
| AP1465d-ev20 | 20 ug 100 ul | In Stock | $ 95.00 | DISCONTINED INQUIRE CLICK INQUIRE Add to cart |
- Specification
- Citiations : 0
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- Backgrounds
ALDH1A1 Antibody - Product info | |
| Application | IF, WB, IHC, FC
|
| Primary Accession | P00352 |
| Reactivity | Human |
| Concentration | 0.25 mg/ml |
| Isotype | Rabbit Ig |
| Calculated MW | 54862 Da |
ALDH1A1 Antibody - Additional info | |
| Gene ID 216 | |
| Other Names ALDH1A1; ALDC; ALDH1; PUMB1; Retinal dehydrogenase 1; ALDH-E1; ALHDII; Aldehyde dehydrogenase family 1 member A1; Aldehyde dehydrogenase, cytosolic | |
| Target/Specificity This ALDH1A1 antibody is generated from rabbits immunized with human ALDH1A1 recombinant protein. | |
| Dilution IF~~1:100 WB~~1:100~500WB~~1:1000 IHC~~1:50~100 FC~~1:10~50 | |
| Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. | |
| Storage Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. | |
| Precautions ALDH1A1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures. | |
ALDH1A1 Antibody - Protein Information | |
| Name ALDH1A1 | |
| Synonyms ALDC, ALDH1, PUMB1 | |
| Function Binds free retinal and cellular retinol-binding protein- bound retinal. Can convert/oxidize retinaldehyde to retinoic acid (By similarity) | |
| Cellular Location Cytoplasm. | |
ALDH1A1 Antibody - Related products
AM1846a: ALDH1A1 Antibody (Ascites)
AP1445a: ALDH1A1 Antibody (N-term)
AP1465c: ALDH1A1 Antibody (Center)
RI10225: ALDH1A1 predesign siRNA
LY12320a: AL1A1 Over-expression Lysate
BP1445a: ALDH1A1 Antibody (N-term) Blocking Peptide
BP1465c: ALDH1A1 Antibody (Center) Blocking Peptide
ALDH1A1 Antibody - Application data
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Fluorescent confocal image of HepG2 cells stained with ALDH1A1 antibody. HepG2 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with AP1465d ALDH1A1 primary antibody (1:100, 2 h at room temperature). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 μg/ml, 5 min). ALDH1A1 immunoreactivity is localized to the cytoplasm of HepG2 cells.
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Western blot analysis of ALDH1A1 Antibody (Cat. #AP1465d) in NCI-H460, HepG2 cell line lysates (35ug/lane). ALDH1A1 (arrow) was detected using the purified Pab.
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ALDH1A1 Antibody (Cat. #AP1465d) IHC analysis in formalin fixed and paraffin embedded human hepatocarcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the ALDH1A1 Antibody for immunohistochemistry. Clinical relevance has not been evaluated.
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ALDH1A1 Antibody (Cat. #AP1465d) flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
ALDH1A1 Antibody - Research Areas
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BACKGROUND
ALDH1A1 belongs to the aldehyde dehydrogenases family of proteins. Aldehyde dehydrogenase is the second enzyme of the major oxidative pathway of alcohol metabolism. Two major liver isoforms of this enzyme, cytosolic and mitochondrial, can be distinguished by their electrophoretic mobilities, kinetic properties, and subcellular localizations. Most Caucasians have two major isozymes, while approximately 50% of Orientals have only the cytosolic isozyme, missing the mitochondrial isozyme. A remarkably higher frequency of acute alcohol intoxication among Orientals than among Caucasians could be related to the absence of the mitochondrial isozyme.
REFERENCES
References for protein: 1.Moore,S., J Stud Alcohol Drugs 68 (2), 192-196 (2007) 2.Collard,F., Biochimie 89 (3), 369-373 (2007) References for HepG2 cell line: 1. Knowles BB, et al. (1980). Human hepatocellular carcinoma cell lines secrete the major plasma proteins and hepatitis B surface antigen. Science 209: 497-499.[ PubMed: 6248960]. 2. Darlington GJ, et al. (1987). Growth and hepatospecific gene expression of human hepatoma cells in a defined medium. In Vitro Cell. Dev. Biol. 23: 349-354.[PubMed: 3034851]. 3. Ihrke, G; Neufeld, EB; Meads, T; Shanks, MR; Cassio, D; Laurent, M; Schroer, TA; Pagano, RE et al. (1993). "WIF-B cells: an in vitro model for studies of hepatocyte polarity". Journal of Cell Biology 123 (6): 1761–1775. [PubMed:7506266]. 4. Mersch-Sundermann, V.; Knasmüller, S.; Wu, X. J.; Darroudi, F.; Kassie, F. (2004). "Use of a human-derived liver cell line for the detection of cytoprotective, antigenotoxic and cogenotoxic agents". Toxicology 198 (1–3): 329–340. [PubMed:15138059].
