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RUNX3 Antibody (Center)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

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  • WB - RUNX3 Antibody (Center) AP14667c
    Western blot analysis of lysates from HL-60, Jurkat cell line (from left to right), using RUNX3 Antibody (Center) (Cat. #AP14667c). AP14667c was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.
  • IHC-P - RUNX3 Antibody (Center) AP14667c
    RUNX3 Antibody (Center) (AP14667c)immunohistochemistry analysis in formalin fixed and paraffin embedded human stomach tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of RUNX3 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
  • IF - RUNX3 Antibody (Center) AP14667c
    Fluorescent confocal image of Hela cell stained with RUNX3 Antibody (Center)(Cat#AP14667c).Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with RUNX3 primary antibody (1:25, 1 h at 37℃). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37℃).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37℃). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min). RUNX3 immunoreactivity is localized to Nucleus and Cytoplasm significantly.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q13761
Other Accession NP_001026850.1, NP_004341.1
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 44356 Da
Antigen Region 168-197 aa
Additional Information
Gene ID 864
Other Names Runt-related transcription factor 3, Acute myeloid leukemia 2 protein, Core-binding factor subunit alpha-3, CBF-alpha-3, Oncogene AML-2, Polyomavirus enhancer-binding protein 2 alpha C subunit, PEA2-alpha C, PEBP2-alpha C, SL3-3 enhancer factor 1 alpha C subunit, SL3/AKV core-binding factor alpha C subunit, RUNX3, AML2, CBFA3, PEBP2A3
Target/Specificity This RUNX3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 168-197 amino acids from the Central region of human RUNX3.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsRUNX3 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name RUNX3
Synonyms AML2, CBFA3, PEBP2A3
Function CBF binds to the core site, 5'-PYGPYGGT-3', of a number of enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers, lck, IL-3 and GM-CSF promoters.
Cellular Location Nucleus {ECO:0000255|PROSITE- ProRule:PRU00399, ECO:0000269|PubMed:20100835}. Cytoplasm. Note=The tyrosine phosphorylated form localizes to the cytoplasm
Research Areas
Citations ( 0 )


This gene encodes a member of the runt domain-containing family of transcription factors. A heterodimer of this protein and a beta subunit forms a complex that binds to the core DNA sequence 5'-PYGPYGGT-3' found in a number of enhancers and promoters, and can either activate or suppress transcription. It also interacts with other transcription factors. It functions as a tumor suppressor, and the gene is frequently deleted or transcriptionally silenced in cancer. Multiple transcript variants encoding different isoforms have been found for this gene.


Tsang, Y.H., et al. Oncogene 29(41):5643-5650(2010)
Shimada, M., et al. Hum. Genet. 128(4):433-441(2010)
Iwatani, K., et al. Biochem. Biophys. Res. Commun. 400(3):426-431(2010)
Kodach, L.L., et al. Carcinogenesis 31(9):1567-1575(2010)
Nishio, M., et al. Anticancer Res. 30(7):2673-2682(2010)

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$ 295.00
$ 99.00
Cat# AP14667c
(40 western blots)
Availability: In Stock
Bulk Size
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