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UBE4A Antibody (Center)
Affinity Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| IHC-P, WB, E |
|---|---|
| Primary Accession | Q14139 |
| Other Accession | A5PKG6, NP_004779.2 |
| Reactivity | Human |
| Predicted | Bovine |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Calculated MW | 122561 Da |
| Antigen Region | 451-479 aa |
| Gene ID | 9354 |
|---|---|
| Other Names | Ubiquitin conjugation factor E4 A, 632-, UBE4A (HGNC:12499) |
| Target/Specificity | This UBE4A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 451-479 amino acids from the Central region of human UBE4A. |
| Dilution | WB~~1:1000 IHC-P~~1:10~50 |
| Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | UBE4A Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | UBE4A (HGNC:12499) |
|---|---|
| Function | Ubiquitin-protein ligase that probably functions as an E3 ligase in conjunction with specific E1 and E2 ligases. May also function as an E4 ligase mediating the assembly of polyubiquitin chains on substrates ubiquitinated by another E3 ubiquitin ligase. Mediates 'Lys-48'-linked polyubiquitination of substrates. |
| Cellular Location | Cytoplasm {ECO:0000250|UniProtKB:E9Q735}. |
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Provided below are standard protocols that you may find useful for product applications.
Background
The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes an additional conjugation factor, E4, which is involved in multiubiquitin chain assembly.
References
Shi, D., et al. Proc. Natl. Acad. Sci. U.S.A. 106(38):16275-16280(2009)
Sakiyama, T., et al. Inflamm. Bowel Dis. 14(3):310-317(2008)
Yang, Y., et al. Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi 23(6):504-506(2007)
Ewing, R.M., et al. Mol. Syst. Biol. 3, 89 (2007) :
Spinette, S., et al. Cell Cycle 3(12):1638-1644(2004)
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