|Application ||WB, E|
|Other Accession||NP_001073860.1, NP_003104.2|
|Calculated MW||100417 Da|
|Antigen Region||199-227 aa|
|Other Names||Nuclear autoantigen Sp-100, Nuclear dot-associated Sp100 protein, Speckled 100 kDa, SP100|
|Target/Specificity||This SP100 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 199-227 amino acids from the N-terminal region of human SP100.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||SP100 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Together with PML, this tumor suppressor is a major constituent of the PML bodies, a subnuclear organelle involved in a large number of physiological processes including cell growth, differentiation and apoptosis. Functions as a transcriptional coactivator of ETS1 and ETS2 according to PubMed:11909962. Under certain conditions, it may also act as a corepressor of ETS1 preventing its binding to DNA according to PubMed:15247905. Through the regulation of ETS1 it may play a role in angiogenesis, controlling endothelial cell motility and invasion. Through interaction with the MRN complex it may be involved in the regulation of telomeres lengthening. May also regulate TP53- mediated transcription and through CASP8AP2, regulate FAS-mediated apoptosis. Also plays a role in infection by viruses, including human cytomegalovirus and Epstein-Barr virus, through mechanisms that may involve chromatin and/or transcriptional regulation.|
|Cellular Location||Nucleus. Nucleus, PML body. Cytoplasm. Note=Differences in the subnuclear localization of the different isoforms seem to exist and may also be cell cycle- and interferon- dependent. Accumulates in the cytoplasm upon FAS activation|
|Tissue Location||Widely expressed. Sp100-B is expressed only in spleen, tonsil, thymus, mature B-cell line and some T-cell line, but not in brain, liver, muscle or non-lymphoid cell lines|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
SP100 may play a role in the control of gene expression.
Shimada, M., et al. Hum. Genet. 128(4):433-441(2010)
Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010) :
Cirulli, E.T., et al. Eur. J. Hum. Genet. 18(7):815-820(2010)
Li, W., et al. Med. Sci. Monit. 16 (6), BR174-BR178 (2010) :
Lang, M., et al. J. Cell. Sci. 123 (PT 3), 392-400 (2010) :
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