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TCEA1 Antibody (Center)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - TCEA1 Antibody (Center) AP14761c
    TCEA1 Antibody (Center) (Cat. #AP14761c) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the TCEA1 antibody detected the TCEA1 protein (arrow).
  • IHC-P - TCEA1 Antibody (Center) AP14761c
    TCEA1 Antibody (Center) (AP14761c)immunohistochemistry analysis in formalin fixed and paraffin embedded human tonsil tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of TCEA1 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
  • SPECIFICATION
  • CITATIONS
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, E
Primary Accession P23193
Other Accession NP_006747.1, NP_958845.1
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 33970 Da
Antigen Region 98-126 aa
Additional Information
Gene ID 6917
Other Names Transcription elongation factor A protein 1, Transcription elongation factor S-II protein 1, Transcription elongation factor TFIISo, TCEA1, GTF2S, TFIIS
Target/Specificity This TCEA1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 98-126 amino acids from the Central region of human TCEA1.
Dilution WB~~1:1000
IHC-P~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsTCEA1 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name TCEA1
Synonyms GTF2S, TFIIS
Function Necessary for efficient RNA polymerase II transcription elongation past template-encoded arresting sites. The arresting sites in DNA have the property of trapping a certain fraction of elongating RNA polymerases that pass through, resulting in locked ternary complexes. Cleavage of the nascent transcript by S-II allows the resumption of elongation from the new 3'-terminus.
Cellular Location Nucleus.
Citations (0)

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Background

Necessary for efficient RNA polymerase II transcription elongation past template-encoded arresting sites. The arresting sites in DNA have the property of trapping a certain fraction of elongating RNA polymerases that pass through, resulting in locked ternary complexes. Cleavage of the nascent transcript by S-II allows the resumption of elongation from the new 3'-terminus.

References

Sugiyama, N., et al. Mol. Cell Proteomics 6(6):1103-1109(2007)
Matsuoka, S., et al. Science 316(5828):1160-1166(2007)
Olsen, J.V., et al. Cell 127(3):635-648(2006)
Olsen, J.V., et al. Cell 127(3):635-648(2006)
Asp, J., et al. Genes Chromosomes Cancer 45(9):820-828(2006)

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$ 295.00
$ 99.00
Cat# AP14761c
Size:
Quantity:
(40 western blots)
Availability: In Stock
Bulk Size
Seasonal Special on Bulk Order
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