|Application ||WB, E|
|Other Accession||Q08464, Q9JIP6, NP_001457.1|
|Calculated MW||63554 Da|
|Antigen Region||534-563 aa|
|Other Names||Frizzled-2, Fz-2, hFz2, FzE2, FZD2|
|Target/Specificity||This FZD2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 534-563 amino acids from the C-terminal region of human FZD2.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||FZD2 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Receptor for Wnt proteins. Most of frizzled receptors are coupled to the beta-catenin canonical signaling pathway, which leads to the activation of disheveled proteins, inhibition of GSK- 3 kinase, nuclear accumulation of beta-catenin and activation of Wnt target genes. A second signaling pathway involving PKC and calcium fluxes has been seen for some family members, but it is not yet clear if it represents a distinct pathway or if it can be integrated in the canonical pathway, as PKC seems to be required for Wnt-mediated inactivation of GSK-3 kinase. Both pathways seem to involve interactions with G-proteins. May be involved in transduction and intercellular transmission of polarity information during tissue morphogenesis and/or in differentiated tissues.|
|Cellular Location||Membrane; Multi-pass membrane protein. Cell membrane; Multi-pass membrane protein|
|Tissue Location||Widely expressed. In the adult, mainly found in heart, placenta, skeletal muscle, lung, kidney, pancreas, prostate, testis, ovary and colon. In the fetus, expressed in brain, lung and kidney. Low levels in fetal liver|
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Provided below are standard protocols that you may find useful for product applications.
Members of the 'frizzled' gene family encode 7-transmembrane domain proteins that are receptors for Wnt signaling proteins. The expression of the FZD2 gene appears to be developmentally regulated, with high levels of expression in fetal kidney and lung and in adult colon and ovary.
Bazhin, A.V., et al. Cell. Mol. Life Sci. 67(5):817-828(2010)
Sato, A., et al. EMBO J. 29(1):41-54(2010)
Jugessur, A., et al. PLoS ONE 5 (7), E11493 (2010) :
Ollila, H.M., et al. Mol. Psychiatry 14(4):351-353(2009)
Wang, H.X., et al. Mol. Hum. Reprod. 15(1):11-17(2009)
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