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Mouse Eef2k Antibody (N-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

  • WB - Mouse Eef2k Antibody (N-term) AP14838a
    Anti-Mouse Eef2k Antibody (N-term) at 1:1000 dilution + NIH/3T3 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 82 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession O08796
Other Accession NP_031934.1
Reactivity Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 81739 Da
Antigen Region 178-205 aa
Additional Information
Gene ID 13631
Other Names Eukaryotic elongation factor 2 kinase, eEF-2 kinase, eEF-2K, Calcium/calmodulin-dependent eukaryotic elongation factor 2 kinase, Eef2k
Target/Specificity This Mouse Eef2k antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 178-205 amino acids from the N-terminal region of mouse Eef2k.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsMouse Eef2k Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name Eef2k
Function Threonine kinase that regulates protein synthesis by controlling the rate of peptide chain elongation. Upon activation by a variety of upstream kinases including AMPK or TRPM7, phosphorylates the elongation factor EEF2 at a single site, renders it unable to bind ribosomes and thus inactive. In turn, the rate of protein synthesis is reduced.
Tissue Location Ubiquitously expressed. Particularly abundant in skeletal muscle and heart
Research Areas
Citations (0)

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Phosphorylates eukaryotic elongation factor-2. Binds calmodulin (By similarity).


Khan, A.A., et al. Am. J. Pathol. 177(1):481-492(2010)
Shen, Q., et al. Parkinsonism Relat. Disord. 15(7):525-531(2009)
Py, B.F., et al. Autophagy 5(3):393-396(2009)
Im, H.I., et al. PLoS ONE 4 (10), E7424 (2009) :
Crawley, S.W., et al. Biochim. Biophys. Acta 1784(6):908-915(2008)

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$ 295.00
$ 99.00
Cat# AP14838a
(40 western blots)
Availability: In Stock
Bulk Size
Seasonal Special on Bulk Order
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