|Application ||WB, E|
|Calculated MW||81739 Da|
|Antigen Region||178-205 aa|
|Other Names||Eukaryotic elongation factor 2 kinase, eEF-2 kinase, eEF-2K, Calcium/calmodulin-dependent eukaryotic elongation factor 2 kinase, Eef2k|
|Target/Specificity||This Mouse Eef2k antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 178-205 amino acids from the N-terminal region of mouse Eef2k.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||Mouse Eef2k Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Threonine kinase that regulates protein synthesis by controlling the rate of peptide chain elongation. Upon activation by a variety of upstream kinases including AMPK or TRPM7, phosphorylates the elongation factor EEF2 at a single site, renders it unable to bind ribosomes and thus inactive. In turn, the rate of protein synthesis is reduced.|
|Tissue Location||Ubiquitously expressed. Particularly abundant in skeletal muscle and heart|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
Phosphorylates eukaryotic elongation factor-2. Binds calmodulin (By similarity).
Khan, A.A., et al. Am. J. Pathol. 177(1):481-492(2010)
Shen, Q., et al. Parkinsonism Relat. Disord. 15(7):525-531(2009)
Py, B.F., et al. Autophagy 5(3):393-396(2009)
Im, H.I., et al. PLoS ONE 4 (10), E7424 (2009) :
Crawley, S.W., et al. Biochim. Biophys. Acta 1784(6):908-915(2008)
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