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SPI1 Antibody (C-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

  • IF - SPI1 Antibody (C-term) AP14850B
    Fluorescent image of NIH/3T3 cell stained with SPI1 Antibody (C-term)(Cat#AP14850b/SA110831AS).NIH/3T3 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with SPI1 primary antibody (1:25, 1 h at 37℃). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37℃).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37℃). SPI1 immunoreactivity is localized to Nucleus significantly.
  • WB - SPI1 Antibody (C-term) AP14850B
    SPI1 Antibody (C-term) (Cat. #AP14850b) western blot analysis in WiDr cell line lysates (35ug/lane).This demonstrates the SPI1 antibody detected the SPI1 protein (arrow).
  • IHC-P - SPI1 Antibody (C-term) AP14850B
    SPI1 Antibody (C-term) (AP14850b)immunohistochemistry analysis in formalin fixed and paraffin embedded human tonsil tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of SPI1 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P17947
Other Accession Q6BDS1, Q6PKU1, P17433, NP_003111.2, NP_001074016.1
Reactivity Human
Predicted Mouse, Pig, Rat
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 31083 Da
Antigen Region 217-246 aa
Additional Information
Gene ID 6688
Other Names Transcription factor PU1, 31 kDa-transforming protein, SPI1
Target/Specificity This SPI1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 217-246 amino acids from the C-terminal region of human SPI1.
Dilution IF~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsSPI1 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name SPI1
Function Binds to the PU-box, a purine-rich DNA sequence (5'- GAGGAA-3') that can act as a lymphoid-specific enhancer. This protein is a transcriptional activator that may be specifically involved in the differentiation or activation of macrophages or B- cells. Also binds RNA and may modulate pre-mRNA splicing (By similarity).
Cellular Location Nucleus {ECO:0000255|PROSITE- ProRule:PRU00237, ECO:0000269|PubMed:23166356}
Research Areas
Citations (0)

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This gene encodes an ETS-domain transcription factor that activates gene expression during myeloid and B-lymphoid cell development. The nuclear protein binds to a purine-rich sequence known as the PU-box found near the promoters of target genes, and regulates their expression in coordination with other transcription factors and cofactors. The protein can also regulate alternative splicing of target genes. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq].


Rimmele, P., et al. Cancer Res. 70(17):6757-6766(2010)
Bonadies, N., et al. Oncogene 29(7):1062-1072(2010)
Bonadies, N., et al. Blood 115(2):331-334(2010)
Desai, S., et al. J. Immunol. 183(9):5778-5787(2009)
Burda, P., et al. Mol. Cancer Res. 7(10):1693-1703(2009)

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Cat# AP14850B
(40 western blots)
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