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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   NANOG Antibody (Center)   

NANOG Antibody (Center)

Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - NANOG Antibody (Center) AP1486c
    NANOG Antibody (Center) (Cat. #AP1486c) western blot analysis in MCF-7 cell line lysates (35ug/lane).This demonstrates the NANOG antibody detected the NANOG protein (arrow).
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  • CITATIONS: 1
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, E
Primary Accession Q9H9S0
Other Accession Q6NSW7, Q5TM84, Q4JM65, Q8N7R0
Reactivity Human
Predicted Bovine, Monkey
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 34620 Da
Antigen Region 94-123 aa
Additional Information
Gene ID 79923
Other Names Homeobox protein NANOG, Homeobox transcription factor Nanog, hNanog, NANOG
Target/Specificity This NANOG antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 94-123 amino acids from the Central region of human NANOG.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsNANOG Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name NANOG
Function Transcription regulator involved in inner cell mass and embryonic stem (ES) cells proliferation and self-renewal. Imposes pluripotency on ES cells and prevents their differentiation towards extraembryonic endoderm and trophectoderm lineages. Blocks bone morphogenetic protein-induced mesoderm differentiation of ES cells by physically interacting with SMAD1 and interfering with the recruitment of coactivators to the active SMAD transcriptional complexes. Acts as a transcriptional activator or repressor. Binds optimally to the DNA consensus sequence 5'-TAAT[GT][GT]-3' or 5'- [CG][GA][CG]C[GC]ATTAN[GC]-3'. Able to autorepress its expression in differentiating (ES) cells: binds to its own promoter following interaction with ZNF281/ZFP281, leading to recruitment of the NuRD complex and subsequent repression of expression. When overexpressed, promotes cells to enter into S phase and proliferation.
Cellular Location Nucleus {ECO:0000255|PROSITE- ProRule:PRU00108, ECO:0000269|PubMed:15983365}
Tissue Location Expressed in testicular carcinoma and derived germ cell tumors (at protein level). Expressed in fetal gonads, ovary and testis. Also expressed in ovary teratocarcinoma cell line and testicular embryonic carcinoma. Not expressed in many somatic organs and oocytes.
Research Areas
Citations ( 0 )

Background

NANOG is a Ttranscription regulator involved in inner cell mass and embryonic stem (ES) cels proliferation and self-renewal. It imposes pluripotency on ES cells and prevents their differentiation towards extraembryonic endoderm and trophectoderm lineages. This protein blocks bone morphogenetic protein-induced mesoderm differentiation of ES cells by physically interacting with SMAD1 and interfering with the recruitment of coactivators to the active SMAD transcriptional complexes. NANOG acts as a transcriptional activator or repressor. It binds optimally to the DNA consensus sequence 5'-[CG][GA][CG]C[GC]ATTAN[GC]-3'. When overexpressed, this protein promotes cells to enter into S phase and proliferation.

References

Kochupurakkal,B.S., Biochem. Biophys. Res. Commun. 365 (4), 846-850 (2008)
Freberg,C.T., Mol. Biol. Cell 18 (5), 1543-1553 (2007)

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$ 295.00
$ 99.00
Cat# AP1486c
Size:
Quantity:
(40 western blots)
Availability: In Stock
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