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PDLIM1 Antibody (C-term)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - PDLIM1 Antibody (C-term) AP14914b
    PDLIM1 Antibody (C-term) (Cat. #AP14914b) western blot analysis in NCI-H292 cell line lysates (35ug/lane).This demonstrates the PDLIM1 antibody detected the PDLIM1 protein (arrow).
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, E
Primary Accession O00151
Other Accession NP_066272.1
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 36072 Da
Antigen Region 254-282 aa
Additional Information
Gene ID 9124
Other Names PDZ and LIM domain protein 1, C-terminal LIM domain protein 1, Elfin, LIM domain protein CLP-36, PDLIM1, CLIM1, CLP36
Target/Specificity This PDLIM1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 254-282 amino acids from the C-terminal region of human PDLIM1.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsPDLIM1 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name PDLIM1
Synonyms CLIM1, CLP36
Function Cytoskeletal protein that may act as an adapter that brings other proteins (like kinases) to the cytoskeleton (PubMed:10861853). Involved in assembly, disassembly and directioning of stress fibers in fibroblasts. Required for the localization of ACTN1 and PALLD to stress fibers. Required for cell migration and in maintaining cell polarity of fibroblasts (By similarity).
Cellular Location Cytoplasm. Cytoplasm, cytoskeleton. Cytoplasm, myofibril, sarcomere, Z line Note=Associates with actin stress fibers
Tissue Location Strongly expressed in the heart and skeletal muscle, moderately expressed in the spleen, small intestine, colon, placenta, and lung. A lower level expression is seen in liver, thymus, kidney, prostate and pancreas and is not found in the brain, testis, ovary, and peripheral blood leukocytes
Research Areas
Citations (0)
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Background

PDLIM1 is a Cytoskeletal protein that may act as an adapter that brings other proteins (like kinases) to the cytoskeleton.

References

Chapuis, J., et al. Mol. Psychiatry 14(11):1004-1016(2009)
Sugiyama, N., et al. Mol. Cell Proteomics 6(6):1103-1109(2007)
Silverberg, M.S., et al. Eur. J. Hum. Genet. 15(3):328-335(2007)
Miehe, U., et al. Histochem. Cell Biol. 126(4):465-471(2006)
Grupe, A., et al. Am. J. Hum. Genet. 78(1):78-88(2006)

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$ 182.50
$ 70.00
Cat# AP14914b
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