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AMFR Antibody (Center)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

  • WB - AMFR Antibody (Center) AP14958c
    AMFR Antibody (Center) (Cat. #AP14958c) western blot analysis in T47D cell line lysates (35ug/lane).This demonstrates the AMFR antibody detected the AMFR protein (arrow).
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q9UKV5
Other Accession NP_001135.3
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 72996 Da
Antigen Region 371-400 aa
Additional Information
Gene ID 267
Other Names E3 ubiquitin-protein ligase AMFR, 632-, Autocrine motility factor receptor, AMF receptor, RING finger protein 45, gp78, AMFR, RNF45
Target/Specificity This AMFR antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 371-400 amino acids from the Central region of human AMFR.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsAMFR Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Synonyms RNF45
Function E3 ubiquitin-protein ligase that mediates the polyubiquitination of a number of proteins such as CD3D, CYP3A4, CFTR and APOB for proteasomal degradation. Component of a VCP/p97- AMFR/gp78 complex that participates in the final step of endoplasmic reticulum-associated degradation (ERAD). The VCP/p97- AMFR/gp78 complex is involved in the sterol-accelerated ERAD degradation of HMGCR through binding to the HMGCR-INSIG complex at the ER membrane and initiating ubiquitination of HMGCR. The ubiquitinated HMGCR is then released from the ER by the complex into the cytosol for subsequent destruction. Also acts as a scaffold protein to assemble a complex that couples ubiquitination, retranslocation and deglycosylation. Mediates tumor invasion and metastasis as a receptor for the GPI/autocrine motility factor.
Cellular Location Endoplasmic reticulum membrane; Multi-pass membrane protein
Research Areas
Citations (0)

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Autocrine motility factor is a tumor motility-stimulating protein secreted by tumor cells. The protein encoded by this gene is a glycosylated transmembrane protein and a receptor for autocrine motility factor. The receptor, which shows some sequence similarity to tumor protein p53, is localized to the leading and trailing edges of carcinoma cells.


Joshi, B., et al. J. Biol. Chem. 285(12):8830-8839(2010)
Bernardi, K.M., et al. Mol. Biol. Cell 21(1):140-151(2010)
Ballar, P., et al. Int. J. Biochem. Cell Biol. 42(1):167-173(2010)
Shmueli, A., et al. Biochem. Biophys. Res. Commun. 390(3):758-762(2009)
Ying, Z., et al. Hum. Mol. Genet. 18(22):4268-4281(2009)

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$ 295.00
$ 99.00
Cat# AP14958c
(40 western blots)
Availability: In Stock
Bulk Size
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