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CD3G Antibody (N-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

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  • WB - CD3G Antibody (N-term) AP1495a
    CD3G Antibody (N-term) (Cat.# AP1495a) western blot analysis in RPMI8226 cell line lysates (35ug/lane).This demonstrates the CD3G antibody detected the CD3G protein (arrow).
  • IHC-P - CD3G Antibody (N-term) AP1495a
    Formalin-fixed and paraffin-embedded human brain tissue reacted with CD3G antibody (N-term) (Cat.#AP1495a), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
  • FC - CD3G Antibody (N-term) AP1495a
    Flow cytometric analysis of HL-60 cells using CD3G Antibody (N-term) (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P09693
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 20469 Da
Antigen Region 48-76 aa
Additional Information
Gene ID 917
Other Names T-cell surface glycoprotein CD3 gamma chain, T-cell receptor T3 gamma chain, CD3g, CD3G, T3G
Target/Specificity This CD3G antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 48-76 amino acids from the N-terminal region of human CD3G.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsCD3G Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name CD3G
Synonyms T3G
Function The CD3 complex mediates signal transduction.
Cellular Location Membrane; Single-pass type I membrane protein
Research Areas
Citations (0)

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CD3G is the CD3-gamma polypeptide, which together with CD3-epsilon, -delta and -zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T-cell receptor-CD3 complex. This complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. The genes encoding the epsilon, gamma and delta polypeptides are located in the same cluster on chromosome 11. Defects in the CD3G gene are associated with T cell immunodeficiency.


Siegers,G.M., J. Exp. Med. 204 (11), 2537-2544 (2007)
Sigalov,A.B., Biochemistry 45 (51), 15731-15739 (2006)

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Cat# AP1495a
(40 western blots)
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