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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   Mouse TLR6 Antibody (C-term)   

Mouse TLR6 Antibody (C-term)

Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - Mouse TLR6 Antibody (C-term) AP1506c
    Western blot analysis of Mouse TLR6 Antibody (C-term) (Cat.# AP1506c) in K562 cell line lysates (35ug/lane). TLR6 (arrow) was detected using the purified Pab.
  • IHC-P - Mouse TLR6 Antibody (C-term) AP1506c
    Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
  • IHC-P - Mouse TLR6 Antibody (C-term) AP1506c
    Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with Mouse TLR6 antibody (C-term) (Cat.#AP1506c), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, E
Primary Accession Q9EPW9
Reactivity Human, Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 91116 Da
Antigen Region 722-753 aa
Additional Information
Gene ID 21899
Other Names Toll-like receptor 6, CD286, Tlr6
Target/Specificity This Mouse TLR6 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 722-753 amino acids from the C-terminal region of mouse TLR6.
Dilution WB~~1:1000
IHC-P~~1:50~100
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsMouse TLR6 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name Tlr6
Function Participates in the innate immune response to Gram- positive bacteria and fungi. Specifically recognizes diacylated and, to a lesser extent, triacylated lipopeptides (PubMed:19931471). In response to diacylated lipopeptides, forms the activation cluster TLR2:TLR6:CD14:CD36, this cluster triggers signaling from the cell surface and subsequently is targeted to the Golgi in a lipid-raft dependent pathway. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Recognizes mycoplasmal macrophage- activating lipopeptide-2kD (MALP-2), soluble tuberculosis factor (STF), phenol-soluble modulin (PSM) and B.burgdorferi outer surface protein A lipoprotein (OspA-L) cooperatively with TLR2. In complex with TLR4, promotes sterile inflammation in monocytes/macrophages in response to oxidized low-density lipoprotein (oxLDL) or amyloid-beta 42. In this context, the initial signal is provided by oxLDL- or amyloid-beta 42-binding to CD36. This event induces the formation of a heterodimer of TLR4 and TLR6, which is rapidly internalized and triggers inflammatory response, leading to the NF-kappa-B-dependent production of CXCL1, CXCL2 and CCL9 cytokines, via MYD88 signaling pathway, and CCL5 cytokine, via TICAM1 signaling pathway, as well as IL1B secretion (PubMed:20037584, PubMed:23812099).
Cellular Location Cell membrane; Single-pass type I membrane protein. Cytoplasmic vesicle, phagosome membrane; Single- pass type I membrane protein. Membrane raft {ECO:0000250|UniProtKB:Q9Y2C9}. Golgi apparatus {ECO:0000250|UniProtKB:Q9Y2C9}. Note=Upon complex formation with CD36 and TLR4, internalized through dynamin-dependent endocytosis Does not reside in lipid rafts before stimulation but accumulates increasingly in the raft upon the presence of the microbial ligand. In response to diacylated lipoproteins, TLR2:TLR6 heterodimers are recruited in lipid rafts, this recruitment determine the intracellular targeting to the Golgi apparatus {ECO:0000250|UniProtKB:Q9Y2C9}
Tissue Location Detected in thymus, spleen, ovary and lung. Expressed in macrohpages.
Research Areas
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Background

TLR6, a Type I membrane protein that belongs to the Toll-like rceptor family, participates in the innate immune response to Gram-positive bacteria and fungi. It acts via MyD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. The protein recognizes mycoplasmal macrophage-activating lipopeptide-2kD (MALP-2), soluble tuberculosis factor (STF), phenol-soluble modulin (PSM) and B.burgdorferi outer surface protein A lipoprotein (OspA-L) cooperatively with TLR2. It binds to TLR2 via their respective extracellular domains, and to MyD88 via their respective TIR domains. TLR6 is detected in monocytes, CD11c+ immature dendritic cells, plasmacytoid pre-dendritic cells and dermal microvessel endothelial cells.

References

Ozinsky, A., et al., Proc. Natl. Acad. Sci. U.S.A. 97(25):13766-13771 (2000).
Takeuchi, O., et al., Gene 231 (1-2), 59-65 (1999).

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Cat# AP1506c
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