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VILIP1 Antibody (C-term)

Purified Rabbit Polyclonal Antibody (Pab)

     
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  • WB - VILIP1 Antibody (C-term) AP1561a
    Western blot analysis of lysates from human brain, mouse brain, rat brain and rat eyeball tissue lysate (from left to right), using VILIP1 Antibody (C-term)(Cat. #AP1561a). AP1561a was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.
  • WB - VILIP1 Antibody (C-term) AP1561a
    Western blot analysis of lysates from SW480 cell line, human brain, mouse brain and rat brain tissue lysate(from left to right), using VILIP1 Antibody (C-term)(Cat. #AP1561a). AP1561a was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.
  • IHC-P - VILIP1 Antibody (C-term) AP1561a
    Formalin-fixed and paraffin-embedded human brain tissue reacted with VILIP1 antibody (C-term ), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
  • IHC-P - VILIP1 Antibody (C-term) AP1561a
    Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with VILIP1 antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, E
Primary Accession P62760
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 22142 Da
Antigen Region 123-150 aa
Additional Information
Gene ID 7447
Other Names Visinin-like protein 1, VILIP, VLP-1, Hippocalcin-like protein 3, HLP3, VSNL1, VISL1
Target/Specificity This VILIP1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 123-150 amino acids from the C-terminal region of human VILIP1.
Dilution WB~~1:1000
IHC-P~~1:50~100
Format Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsVILIP1 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name VSNL1
Synonyms VISL1
Function Regulates (in vitro) the inhibition of rhodopsin phosphorylation in a calcium-dependent manner.
Tissue Location Brain and retina. Neuron-specific in the central and peripheral nervous system. Increased in the cerebrospinal fluid of Alzheimer disease patients (at protein level).
Research Areas
Citations ( 0 )

Background

The visinin and visinin-like peptides represent a family of calcium-binding proteins that are highly expressed in the retina. Visinin has been shown to be a cone cell-specific protein with a molecular weight of 24 kDa. Several members of the visinin family of genes have been isolated and characterized from different species. These peptides are believed to be involved in the processes of phototransduction. The recoverin gene (RCV1) is believed to be involved in the pathophysiology of retinopathy in cancer patients.

References

Braunewell, K.H., et al., Neuropharmacology 44(6):707-715 (2003). Lin, L., et al., J. Biol. Chem. 277(44):41872-41878 (2002). Spilker, C., et al., J. Neurosci. 22(17):7331-7339 (2002). Bernstein, H.G., et al., Neuroreport 13(4):393-396 (2002). Lin, L., et al., Biochem. Biophys. Res. Commun. 296(4):827-832 (2002).

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$ 295.00
$ 99.00
Cat# AP1561a
Size:
Quantity:
(40 western blots)
Availability: In Stock
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