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RAD1 Antibody (Center)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

  • WB - RAD1 Antibody (Center) AP16013c
    RAD1 Antibody (Center) (Cat. #AP16013c) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the RAD1 antibody detected the RAD1 protein (arrow).
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession O60671
Other Accession NP_002844.1
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 31827 Da
Antigen Region 118-146 aa
Additional Information
Gene ID 5810
Other Names Cell cycle checkpoint protein RAD1, hRAD1, DNA repair exonuclease rad1 homolog, Rad1-like DNA damage checkpoint protein, RAD1, REC1
Target/Specificity This RAD1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 118-146 amino acids from the Central region of human RAD1.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsRAD1 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name RAD1
Synonyms REC1
Function Component of the 9-1-1 cell-cycle checkpoint response complex that plays a major role in DNA repair. The 9-1-1 complex is recruited to DNA lesion upon damage by the RAD17-replication factor C (RFC) clamp loader complex. Acts then as a sliding clamp platform on DNA for several proteins involved in long-patch base excision repair (LP-BER). The 9-1-1 complex stimulates DNA polymerase beta (POLB) activity by increasing its affinity for the 3'-OH end of the primer-template and stabilizes POLB to those sites where LP-BER proceeds; endonuclease FEN1 cleavage activity on substrates with double, nick, or gap flaps of distinct sequences and lengths; and DNA ligase I (LIG1) on long-patch base excision repair substrates. The 9-1-1 complex is necessary for the recruitment of RHNO1 to sites of double-stranded breaks (DSB) occurring during the S phase. Isoform 1 possesses 3'->5' double stranded DNA exonuclease activity.
Cellular Location Nucleus.
Tissue Location Expressed in testis, uterus, bladder, spleen, ovaries, lung, brain and muscle (at protein level)
Citations (0)

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This gene encodes a component of a heterotrimeric cell cycle checkpoint complex, known as the 9-1-1 complex, that is activated to stop cell cycle progression in response to DNA damage or incomplete DNA replication. The 9-1-1 complex is recruited by RAD17 to affected sites where it may attract specialized DNA polymerases and other DNA repair effectors. Alternatively spliced transcript variants of this gene have been described. [provided by RefSeq].


Shimada, M., et al. Hum. Genet. 128(4):433-441(2010)
Takeishi, Y., et al. Genes Cells 15(7):761-771(2010)
Bai, H., et al. DNA Repair (Amst.) 9(5):478-487(2010)
Park, M.J., et al. DNA Repair (Amst.) 8(10):1190-1200(2009)
Xu, M., et al. J. Biol. Chem. 284(31):20457-20461(2009)

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$ 295.00
$ 99.00
Cat# AP16013c
(40 western blots)
Availability: In Stock
Bulk Size
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