|Application ||WB, E|
|Other Accession||P60522, P60521, P60519, NP_009216.1|
|Calculated MW||13667 Da|
|Antigen Region||1-28 aa|
|Other Names||Gamma-aminobutyric acid receptor-associated protein-like 2, GABA(A) receptor-associated protein-like 2, Ganglioside expression factor 2, GEF-2, General protein transport factor p16, Golgi-associated ATPase enhancer of 16 kDa, GATE-16, MAP1 light chain 3-related protein, GABARAPL2, FLC3A, GEF2|
|Target/Specificity||This GATE16 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-28 amino acids from the N-terminal region of human GATE16.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||GATE16 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Ubiquitin-like modifier involved in intra-Golgi traffic. Modulates intra-Golgi transport through coupling between NSF activity and SNAREs activation. It first stimulates the ATPase activity of NSF which in turn stimulates the association with GOSR1 (By similarity). Involved in autophagy. Plays a role in mitophagy which contributes to regulate mitochondrial quantity and quality by eliminating the mitochondria to a basal level to fulfill cellular energy requirements and preventing excess ROS production. Whereas LC3s are involved in elongation of the phagophore membrane, the GABARAP/GATE-16 subfamily is essential for a later stage in autophagosome maturation.|
|Cellular Location||Golgi apparatus. Cytoplasmic vesicle, autophagosome|
|Tissue Location||Ubiquitous. Expressed at high levels in the brain, heart, prostate, ovary, spleen and skeletal muscle Expressed at very low levels in lung, thymus and small intestine|
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Provided below are standard protocols that you may find useful for product applications.
Involved in intra-Golgi traffic. Modulates intra-Golgi transport through coupling between NSF activity and SNAREs activation. It first stimulates the ATPase activity of NSF which in turn stimulates the association with GOSR1 (By similarity).
Pinheiro, A.P., et al. Am. J. Med. Genet. B Neuropsychiatr. Genet. 153B (5), 1070-1080 (2010) :
Dastani, Z., et al. Eur. J. Hum. Genet. 18(3):342-347(2010)
Thielmann, Y., et al. Proteins 77(3):637-646(2009)
Kirkin, V., et al. Mol. Cell 33(4):505-516(2009)
Shvets, E., et al. Autophagy 4(8):1054-1056(2008)
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