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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   Mouse Hck Antibody (N-term)   

Mouse Hck Antibody (N-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

  • WB - Mouse Hck Antibody (N-term) AP16107A
    Mouse Hck Antibody (N-term) (Cat. #AP16107a) western blot analysis in CEM cell line lysates (35ug/lane).This demonstrates the Hck antibody detected the Hck protein (arrow).
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P08103
Other Accession P50545, NP_034537.2, NP_001165588.1
Reactivity Human
Predicted Rat
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 59129 Da
Antigen Region 72-101 aa
Additional Information
Gene ID 15162
Other Names Tyrosine-protein kinase HCK, B-cell/myeloid kinase, BMK, Hematopoietic cell kinase, Hemopoietic cell kinase, p56-HCK/p59-HCK, Hck
Target/Specificity This Mouse Hck antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 72-101 amino acids from the N-terminal region of mouse Hck.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsMouse Hck Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name Hck
Function Non-receptor tyrosine-protein kinase found in hematopoietic cells that transmits signals from cell surface receptors and plays an important role in the regulation of innate immune responses, including neutrophil, monocyte, macrophage and mast cell functions, phagocytosis, cell survival and proliferation, cell adhesion and migration. Acts downstream of receptors that bind the Fc region of immunoglobulins, such as FCGR1A and FCGR2A, but also CSF3R, PLAUR, the receptors for IFNG, IL2, IL6 and IL8, and integrins, such as ITGB1 and ITGB2. During the phagocytic process, mediates mobilization of secretory lysosomes, degranulation, and activation of NADPH oxidase to bring about the respiratory burst. Plays a role in the release of inflammatory molecules. Promotes reorganization of the actin cytoskeleton and actin polymerization, formation of podosomes and cell protrusions. Inhibits TP73-mediated transcription activation and TP73-mediated apoptosis. Phosphorylates CBL in response to activation of immunoglobulin gamma Fc region receptors. Phosphorylates ADAM15, BCR, ELMO1, FCGR2A, GAB1, GAB2, RAPGEF1, STAT5B, TP73, VAV1 and WAS (By similarity).
Cellular Location Cytoplasmic vesicle, secretory vesicle. Cytoplasm, cytosol Isoform 2: Cell membrane; Lipid-anchor. Membrane, caveola; Lipid-anchor. Cell junction, focal adhesion. Cytoplasm, cytoskeleton. Golgi apparatus. Cytoplasmic vesicle. Lysosome. Nucleus Note=20% of this isoform is associated with caveolae. Localization at the cell membrane and at caveolae requires palmitoylation at Cys-3. Colocalizes with the actin cytoskeleton at focal adhesions (By similarity).
Tissue Location Expressed predominantly in cells of the myeloid and B-lymphoid lineages
Research Areas
Citations (0)

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The protein encoded by this gene is a member of the Src family of tyrosine kinases. This protein is primarily hemopoietic, particularly in cells of the myeloid and B-lymphoid lineages. It may play a role in the innate immune response and the STAT5 signaling pathway. Alternative translation initiation site usage, including a non-AUG (CUG) codon, results in the production of two different isoforms, that have different subcellular localization.


Verollet, C., et al. J. Immunol. 184(12):7030-7039(2010)
Ruschmann, J., et al. Exp. Hematol. 38(5):392-402(2010)
Lowell, C.A., et al. J. Cell Biol. 133(4):895-910(1996)
Lowell, C.A., et al. Blood 87(5):1780-1792(1996)
Avraham, K.B., et al. Genomics 13(2):264-268(1992)

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Cat# AP16107A
(40 western blots)
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