|Application ||WB, E|
|Other Accession||NP_604389.1, NP_003712.1|
|Calculated MW||28102 Da|
|Antigen Region||94-123 aa|
|Other Names||DNA-binding protein RFXANK, Ankyrin repeat family A protein 1, Regulatory factor X subunit B, RFX-B, Regulatory factor X-associated ankyrin-containing protein, RFXANK, ANKRA1, RFXB|
|Target/Specificity||This RFXANK antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 94-123 amino acids from the Central region of human RFXANK.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||RFXANK Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Activates transcription from class II MHC promoters. Activation requires the activity of the MHC class II transactivator (MHC2TA). May regulate other genes in the cell. RFX binds the X1 box of MHC-II promoters. Isoform RFX-B-delta5 is not involved in the positive regulation of MHC class II genes.|
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Provided below are standard protocols that you may find useful for product applications.
Major histocompatibility (MHC) class II molecules are transmembrane proteins that have a central role in development and control of the immune system. The protein encoded by this gene, along with regulatory factor X-associated protein and regulatory factor-5, forms a complex that binds to the X box motif of certain MHC class II gene promoters and activates their transcription. Once bound to the promoter, this complex associates with the non-DNA-binding factor MHC class II transactivator, which controls the cell type specificity and inducibility of MHC class II gene expression. This protein contains ankyrin repeats involved in protein-protein interactions. Mutations in this gene have been linked to bare lymphocyte syndrome type II, complementation group B. Two transcript variants encoding different isoforms have been described for this gene, with only one isoform showing activation activity.
Garvie, C.W., et al. Biochim. Biophys. Acta 1779(12):797-804(2008)
Ewing, R.M., et al. Mol. Syst. Biol. 3, 89 (2007) :
Krawczyk, M., et al. Mol. Cell. Biol. 25(19):8607-8618(2005)
Wang, A.H., et al. J. Biol. Chem. 280(32):29117-29127(2005)
Grimwood, J., et al. Nature 428(6982):529-535(2004)
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