Mouse Cdk2 Antibody (C-term)Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

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Catalog #	Size	Availability	Price
AP16161b	0.1 mg 400 ul	In Stock	$ 255.00	DISCONTINED INQUIRE CLICK INQUIRE Add to cart
AP16161b-ev20	20 ug 100 ul	In Stock	$ 95.00	DISCONTINED INQUIRE CLICK INQUIRE Add to cart

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Mouse Cdk2 Antibody (C-term) - Product info
Application	WB Applications Legend: W=Western Blotting IP=Immunoprecipitation IHC-P=Immunohistochemistry (Paraffin) IF-IC=Immunofluorescence (Immunocytochemistry) F=Flow Cytometry
Primary Accession	P97377
Other Accession	NP_904326.1, NP_058036.1
Reactivity	Mouse
Concentration	0.25 mg/ml
Isotype	Rabbit Ig
Calculated MW	38978 Da
Mouse Cdk2 Antibody (C-term) - Additional info
Gene ID 12566
Other Names Cdk2; Cdkn2; Cyclin-dependent kinase 2; Cell division protein kinase 2
Target/Specificity This Mouse Cdk2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 226-254 amino acids from the C-terminal region of Mouse Cdk2.
Dilution WB~~1:100~500WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Storage Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Precautions Mouse Cdk2 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Mouse Cdk2 Antibody (C-term) - Protein Information
Name Cdk2
Synonyms Cdkn2
Function Serine/threonine-protein kinase involved in the control of the cell cycle; essential for meiosis, but dispensable for mitosis. Phosphorylates CTNNB1, USP37, p53/TP53, NPM1, CDK7, RB1, BRCA2, MYC, NPAT, EZH2. Interacts with cyclins A, B1, B3, D, or E Triggers duplication of centrosomes and DNA. Acts at the G1-S transition to promote the E2F transcriptional program and the initiation of DNA synthesis, and modulates G2 progression; controls the timing of entry into mitosis/meiosis by controlling the subsequent activation of cyclin B/CDK1 by phosphorylation, and coordinates the activation of cyclin B/CDK1 at the centrosome and in the nucleus. Crucial role in orchestrating a fine balance between cellular proliferation, cell death, and DNA repair in human embryonic stem cells (hESCs). Activity of CDK2 is maximal during S phase and G2; activated by interaction with cyclin E during the early stages of DNA synthesis to permit G1-S transition, and subsequently activated by cyclin A2 (cyclin A1 in germ cells) during the late stages of DNA replication to drive the transition from S phase to mitosis, the G2 phase. EZH2 phosphorylation promotes H3K27me3 maintenance and epigenetic gene silencing. Phosphorylates CABLES1 (By similarity). Cyclin E/CDK2 prevents oxidative stress-mediated Ras-induced senescence by phosphorylating MYC. Involved in G1-S phase DNA damage checkpoint that prevents cells with damaged DNA from initiating mitosis; regulates homologous recombination-dependent repair by phosphorylating BRCA2, this phosphorylation is low in S phase when recombination is active, but increases as cells progress towards mitosis. In response to DNA damage, double-strand break repair by homologous recombination a reduction of CDK2-mediated BRCA2 phosphorylation. Phosphorylation of RB1 disturbs its interaction with E2F1. NPM1 phosphorylation by cyclin E/CDK2 promotes its dissociates from unduplicated centrosomes, thus initiating centrosome duplication. Cyclin E/CDK2-mediated phosphorylation of NPAT at G1-S transition and until prophase stimulates the NPAT- mediated activation of histone gene transcription during S phase Required for vitamin D-mediated growth inhibition by being itself inactivated. Involved in the nitric oxide- (NO) mediated signaling in a nitrosylation/activation-dependent manner. USP37 is activated by phosphorylation and thus triggers G1-S transition. CTNNB1 phosphorylation regulates insulin internalization
Cellular Location Cytoplasm, cytoskeleton, centrosome (By similarity). Nucleus, Cajal body (By similarity). Cytoplasm (By similarity). Endosome (By similarity). Note=Localized at the centrosomes in late G2 phase after separation of the centrosomes but before the start of prophase. Nuclear-cytoplasmic trafficking is mediated during the inhibition by 1,25-(OH)(2)D(3) (By similarity)

Mouse Cdk2 Antibody (C-term) - Related products

AP16161b: Mouse Cdk2 Antibody (C-term)

Mouse Cdk2 Antibody (C-term) - Application data

Mouse Cdk2 Antibody (C-term) (Cat. #AP16161b) western blot analysis in mouse liver tissue lysates (35ug/lane).This demonstrates the Cdk2 antibody detected the Cdk2 protein (arrow).

Mouse Cdk2 Antibody (C-term) - Research Areas

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Provided below are standard protocols that you may find useful for product applications.

BACKGROUND

Cdk2 is involved in the control of the cell cycle. Interacts with cyclins A, B1, B3, D, or E. Activity of CDK2 is maximal during S phase and G2 (By similarity).

REFERENCES

Puyol, M., et al. Cancer Cell 18(1):63-73(2010) Hodeify, R., et al. Am. J. Physiol. Renal Physiol. 299 (1), F112-F120 (2010) : Risley, M.D., et al. Dev. Biol. 342(2):146-156(2010) Copeland, N.A., et al. J. Cell. Sci. 123 (PT 7), 1108-1115 (2010) : Koledova, Z., et al. Stem Cells 28(3):450-461(2010)