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TOP2A Antibody (C-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

  • WB - TOP2A Antibody (C-term) AP16175B
    TOP2A/Topo? Antibody (C-term) (Cat. #AP16175b) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the TOP2A/Topo? antibody detected the TOP2A/Topo? protein (arrow).
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P11388
Other Accession NP_001058.2
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 174385 Da
Antigen Region 1434-1461 aa
Additional Information
Gene ID 7153
Other Names DNA topoisomerase 2-alpha, DNA topoisomerase II, alpha isozyme, TOP2A, TOP2
Target/Specificity This TOP2A/Topo? antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1434-1461 amino acids from the C-terminal region of human TOP2A/Topo?.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsTOP2A Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name TOP2A
Synonyms TOP2
Function Control of topological states of DNA by transient breakage and subsequent rejoining of DNA strands. Topoisomerase II makes double-strand breaks. Essential during mitosis and meiosis for proper segregation of daughter chromosomes. May play a role in regulating the period length of ARNTL/BMAL1 transcriptional oscillation (By similarity).
Cellular Location Cytoplasm. Nucleus, nucleoplasm. Note=Generally located in the nucleoplasm
Research Areas
Citations (0)

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This gene encodes a DNA topoisomerase, an enzyme that controls and alters the topologic states of DNA during transcription. This nuclear enzyme is involved in processes such as chromosome condensation, chromatid separation, and the relief of torsional stress that occurs during DNA transcription and replication. It catalyzes the transient breaking and rejoining of two strands of duplex DNA which allows the strands to pass through one another, thus altering the topology of DNA. Two forms of this enzyme exist as likely products of a gene duplication event. The gene encoding this form, alpha, is localized to chromosome 17 and the beta gene is localized to chromosome 3. The gene encoding this enzyme functions as the target for several anticancer agents and a variety of mutations in this gene have been associated with the development of drug resistance. Reduced activity of this enzyme may also play a role in ataxia-telangiectasia.


Vranic, S., et al. Hum. Pathol. 41(11):1617-1623(2010)
Chen, H., et al. Am. J. Surg. Pathol. 34(9):1250-1257(2010)
Ye, J., et al. Cell 142(2):230-242(2010)
Rossi, E., et al. Histopathology 57(1):81-89(2010)
Sugiyama, N., et al. Mol. Cell Proteomics 6(6):1103-1109(2007)

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$ 295.00
$ 99.00
Cat# AP16175B
(40 western blots)
Availability: In Stock
Bulk Size
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