|Application ||IF, WB, E|
|Other Accession||NP_001028171.1, NP_005084.1|
|Calculated MW||67133 Da|
|Antigen Region||1-29 aa|
|Other Names||Protein CBFA2T2, ETO homologous on chromosome 20, MTG8-like protein, MTG8-related protein 1, Myeloid translocation-related protein 1, p85, CBFA2T2, EHT, MTGR1|
|Target/Specificity||This CBFA2T2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-29 amino acids from the N-terminal region of human CBFA2T2.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||CBFA2T2 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||May function as a complex with the chimeric protein RUNX1/AML1-CBFA2T1/MTG8 which is produced in acute myeloid leukemia with the chromosomal translocation t(8;21). May thus be involved in the repression of AML1-dependent transcription and the induction of G-CSF/CSF3-dependent cell growth. May be a tumor suppressor gene candidate involved in myeloid tumors with the deletion of the 20q11 region.|
|Tissue Location||Ubiquitously expressed in fetal and adult tissues. Highly expressed in adult brain, heart, lung, kidney, lymph node, appendix, thymus, testis, uterus, small intestine, prostate and thymus.|
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Provided below are standard protocols that you may find useful for product applications.
In acute myeloid leukemia, especially in the M2 subtype, the t(8;21)(q22;q22) translocation is one of the most frequent karyotypic abnormalities. The translocation produces a chimeric gene made up of the 5'-region of the RUNX1 (AML1) gene fused to the 3'-region of the CBFA2T1 (MTG8) gene. The chimeric protein is thought to associate with the nuclear corepressor/histone deacetylase complex to block hematopoietic differentiation. The protein encoded by this gene binds to the AML1-MTG8 complex and may be important in promoting leukemogenesis. Several transcript variants are thought to exist for this gene, but the full-length natures of only three have been described.
Guastadisegni, M.C., et al. Leukemia 24(8):1516-1519(2010)
Ossovskaya, V.S., et al. J. Neurosci. Methods 177(2):322-333(2009)
Venkatesan, K., et al. Nat. Methods 6(1):83-90(2009)
Olsson, A., et al. Biochim. Biophys. Acta 1779(10):590-598(2008)
Kumar, R., et al. Mol. Cancer Res. 4(9):655-665(2006)
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