|Application ||WB, E|
|Other Accession||NP_001107019.1, NP_001107018.1|
|Calculated MW||85226 Da|
|Antigen Region||135-163 aa|
|Other Names||LIM domain and actin-binding protein 1, Epithelial protein lost in neoplasm, LIMA1, EPLIN, SREBP3|
|Target/Specificity||This LIMA1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 135-163 amino acids from the N-terminal region of human LIMA1.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||LIMA1 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Binds to actin monomers and filaments. Increases the number and size of actin stress fibers and inhibits membrane ruffling. Inhibits actin filament depolymerization. Bundles actin filaments, delays filament nucleation and reduces formation of branched filaments.|
|Cellular Location||Cytoplasm. Cell junction, focal adhesion. Cytoplasm, cytoskeleton. Note=This cytoskeletal protein co- localizes with actin stress fibers and focal adhesion plaques|
|Tissue Location||Highly expressed in placenta, kidney, pancreas, prostate, ovary, spleen and heart. Also detected in lung, liver, brain, skeletal muscle, thymus, testis and intestine Not detected in leukocytes. Isoform Beta expressed generally at very low levels. Isoform Alpha abundant in epithelial cells from mammary gland, prostate and in normal oral keratinocytes. Low levels in aortic endothelial cells and dermal fibroblasts. Not detectable in myocardium|
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Provided below are standard protocols that you may find useful for product applications.
EPLIN is a cytoskeleton-associated protein that inhibits actin filament depolymerization and cross-links filaments in bundles (Maul et al., 2003 [PubMed 12566430]).
Chircop, M., et al. Cell Cycle 8(5):757-764(2009)
Abe, K., et al. Proc. Natl. Acad. Sci. U.S.A. 105(1):13-19(2008)
Jiang, W.G., et al. Mol. Cancer 7, 71 (2008) :
Sugiyama, N., et al. Mol. Cell Proteomics 6(6):1103-1109(2007)
Olsen, J.V., et al. Cell 127(3):635-648(2006)
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