|Application ||WB, E|
|Other Accession||O08583, Q3T0I4, NP_005773.3|
|Calculated MW||26888 Da|
|Antigen Region||60-89 aa|
|Other Names||THO complex subunit 4, Tho4, Ally of AML-1 and LEF-1, Aly/REF export factor, Transcriptional coactivator Aly/REF, bZIP-enhancing factor BEF, ALYREF, ALY, BEF, THOC4|
|Target/Specificity||This THOC4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 60-89 amino acids from the Central region of human THOC4.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||THOC4 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Synonyms||ALY, BEF, THOC4|
|Function||Export adapter involved in nuclear export of spliced and unspliced mRNA. Binds mRNA which is thought to be transferred to the NXF1-NXT1 heterodimer for export (TAP/NFX1 pathway). Component of the TREX complex which is thought to couple mRNA transcription, processing and nuclear export, and specifically associates with spliced mRNA and not with unspliced pre-mRNA. TREX is recruited to spliced mRNAs by a transcription-independent mechanism, binds to mRNA upstream of the exon-junction complex (EJC) and is recruited in a splicing- and cap-dependent manner to a region near the 5' end of the mRNA where it functions in mRNA export to the cytoplasm. TREX recruitment occurs via an interaction between ALYREF/THOC4 and the cap-binding protein NCBP1. The TREX complex is essential for the export of Kaposi's sarcoma-associated herpesvirus (KSHV) intronless mRNAs and infectious virus production; ALYREF/THOC4 mediates the recruitment of the TREX complex to the intronless viral mRNA. Required for TREX complex assembly and for linking DDX39B to the cap-binding complex (CBC). In conjunction with THOC5 functions in NXF1-NXT1 mediated nuclear export of HSP70 mRNA; both proteins enhance the RNA binding activity of NXF1 and are required for NXF1 localization to the nuclear rim. Involved in the nuclear export of intronless mRNA; proposed to be recruited to intronless mRNA by ATP-bound DDX39B. Involved in transcription elongation and genome stability.|
|Cellular Location||Nucleus. Nucleus speckle Cytoplasm. Note=Colocalizes with the core EJC, ALYREF/THOC4, NXF1 and DDX39B in the nucleus and nuclear speckles. Travels to the cytoplasm as part of the exon junction complex (EJC) bound to mRNA (PubMed:19324961). Localizes to regions surrounding nuclear speckles known as perispeckles in which TREX complex assembly seems to occur (PubMed:23826332)|
|Tissue Location||Expressed in a wide variety of cancer types.|
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Provided below are standard protocols that you may find useful for product applications.
THOC4 is a heat stable, nuclear protein and functions as a molecular chaperone. It is thought to regulate dimerization, DNA binding, and transcriptional activity of basic region-leucine zipper (bZIP) proteins.
Corbin-Lickfett, K.A., et al. J. Virol. 84(5):2212-2222(2010)
Souki, S.K., et al. J. Virol. 83(17):8970-8975(2009)
Johnson, L.A., et al. J. Virol. 83(13):6335-6346(2009)
Colgan, K.J., et al. J. Gen. Virol. 90 (PT 6), 1455-1460 (2009) :
Katahira, J., et al. EMBO J. 28(5):556-567(2009)
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