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KLHL25 Antibody (N-term)
Affinity Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, E |
|---|---|
| Primary Accession | Q9H0H3 |
| Other Accession | Q4KLM4, Q8R2P1, NP_071925.2 |
| Reactivity | Human |
| Predicted | Mouse, Rat |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Calculated MW | 65923 Da |
| Antigen Region | 62-91 aa |
| Gene ID | 64410 |
|---|---|
| Other Names | Kelch-like protein 25, Ectoderm-neural cortex protein 2, ENC-2, KLHL25, ENC2 |
| Target/Specificity | This KLHL25 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 62-91 amino acids from the N-terminal region of human KLHL25. |
| Dilution | WB~~1:1000 |
| Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | KLHL25 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | KLHL25 {ECO:0000303|PubMed:22578813, ECO:0000312|HGNC:HGNC:25732} |
|---|---|
| Function | Substrate-specific adapter of a BCR (BTB-CUL3-RBX1) E3 ubiquitin ligase complex involved in various processes, such as translation homeostasis and lipid synthesis (PubMed:22578813, PubMed:27664236, PubMed:34491895). The BCR(KLHL25) ubiquitin ligase complex acts by mediating ubiquitination of hypophosphorylated EIF4EBP1 (4E-BP1): ubiquitination and subsequent degradation of hypophosphorylated EIF4EBP1 (4E-BP1) probably serves as a homeostatic mechanism to maintain translation and prevent eIF4E inhibition when eIF4E levels are low (PubMed:22578813). The BCR(KLHL25) complex does not target EIF4EBP1 (4E-BP1) when it is hyperphosphorylated or associated with eIF4E (PubMed:22578813). The BCR(KLHL25) complex also acts as a regulator of lipid synthesis by mediating ubiquitination and degradation of ACLY, thereby inhibiting lipid synthesis (PubMed:27664236, PubMed:34491895). BCR(KLHL25)-mediated degradation of ACLY promotes fatty acid oxidation and is required for differentiation of inducible regulatory T (iTreg) cells (PubMed:34491895). |
Research Areas
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Application Protocols
Provided below are standard protocols that you may find useful for product applications.
References
Lunetta, K.L., et al. BMC Med. Genet. 8 SUPPL 1, S13 (2007) :
Simpson, J.C., et al. EMBO Rep. 1(3):287-292(2000)
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$ 182.50
$ 70.00
Cat# AP16430a
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