|Application ||WB, E|
|Other Accession||Q4KLM4, Q8R2P1, NP_071925.2|
|Calculated MW||65923 Da|
|Antigen Region||62-91 aa|
|Other Names||Kelch-like protein 25, Ectoderm-neural cortex protein 2, ENC-2, KLHL25, ENC2|
|Target/Specificity||This KLHL25 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 62-91 amino acids from the N-terminal region of human KLHL25.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||KLHL25 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Substrate-specific adapter of a BCR (BTB-CUL3-RBX1) E3 ubiquitin ligase complex required for translational homeostasis. The BCR(KLHL25) ubiquitin ligase complex acts by mediating ubiquitination of hypophosphorylated EIF4EBP1 (4E-BP1): ubiquitination and subsequent degradation of hypophosphorylated EIF4EBP1 (4E-BP1) probably serves as a homeostatic mechanism to maintain translation and prevent eIF4E inhibition when eIF4E levels are low. The BCR(KLHL25) complex does not target EIF4EBP1 (4E-BP1) when it is hyperphosphorylated or associated with eIF4E.|
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Provided below are standard protocols that you may find useful for product applications.
Lunetta, K.L., et al. BMC Med. Genet. 8 SUPPL 1, S13 (2007) :
Simpson, J.C., et al. EMBO Rep. 1(3):287-292(2000)
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