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TCEA3 Antibody (Center)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - TCEA3 Antibody (Center) AP16463c
    TCEA3 Antibody (Center) (Cat. #AP16463c) western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the TCEA3 antibody detected the TCEA3 protein (arrow).
  • SPECIFICATION
  • CITATIONS
  • PROTOCOLS
  • BACKGROUND
Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, E
Primary Accession O75764
Other Accession NP_003187.1
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 38972 Da
Antigen Region 102-130 aa
Additional Information
Gene ID 6920
Other Names Transcription elongation factor A protein 3, Transcription elongation factor S-II protein 3, Transcription elongation factor TFIISh, TCEA3, TFIISH
Target/Specificity This TCEA3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 102-130 amino acids from the Central region of human TCEA3.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsTCEA3 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name TCEA3
Synonyms TFIISH
Function Necessary for efficient RNA polymerase II transcription elongation past template-encoded arresting sites. The arresting sites in DNA have the property of trapping a certain fraction of elongating RNA polymerases that pass through, resulting in locked ternary complexes. Cleavage of the nascent transcript by S-II allows the resumption of elongation from the new 3'-terminus.
Cellular Location Nucleus {ECO:0000255|PROSITE- ProRule:PRU00649, ECO:0000255|PROSITE-ProRule:PRU00651}
Citations (0)

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Background

TCEA3 is necessary for efficient RNA polymerase II transcription elongation past template-encoded arresting sites. The arresting sites in DNA have the property of trapping a certain fraction of elongating RNA polymerases that pass through, resulting in locked ternary complexes. Cleavage of the nascent transcript by S-II allows the resumption of elongation from the new 3'-terminus.

References

Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010)
Kim, J., et al. Cell 140(4):491-503(2010)
Talmud, P.J., et al. Am. J. Hum. Genet. 85(5):628-642(2009)
Labhart, P., et al. Genomics 52(3):278-288(1998)

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$ 295.00
$ 99.00
Cat# AP16463c
Size:
Quantity:
(40 western blots)
Availability: In Stock
Bulk Size
Seasonal Special on Bulk Order
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