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PHF21A Antibody (Center)
Affinity Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, E |
|---|---|
| Primary Accession | Q96BD5 |
| Other Accession | Q6ZPK0, NP_057705.3 |
| Reactivity | Human |
| Predicted | Mouse |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Calculated MW | 74854 Da |
| Antigen Region | 436-464 aa |
| Gene ID | 51317 |
|---|---|
| Other Names | PHD finger protein 21A, BHC80a, BRAF35-HDAC complex protein BHC80, PHF21A, BHC80, KIAA1696 |
| Target/Specificity | This PHF21A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 436-464 amino acids from the Central region of human PHF21A. |
| Dilution | WB~~1:1000 |
| Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | PHF21A Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | PHF21A |
|---|---|
| Synonyms | BHC80, KIAA1696 |
| Function | Component of the BHC complex, a corepressor complex that represses transcription of neuron-specific genes in non-neuronal cells. The BHC complex is recruited at RE1/NRSE sites by REST and acts by deacetylating and demethylating specific sites on histones, thereby acting as a chromatin modifier. In the BHC complex, it may act as a scaffold. Inhibits KDM1A-mediated demethylation of 'Lys-4' of histone H3 in vitro, suggesting a role in demethylation regulation. |
| Cellular Location | Nucleus. |
| Tissue Location | Highly expressed in brain (PubMed:31649809). Expressed at lower level in other tissues, including heart, kidney, liver, lung and skeletal muscle (PubMed:31649809). Abundantly expressed in fetal brain (PubMed:31649809). |
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abcepta to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
Background
The PHF21A gene encodes BHC80, a component of a BRAF35 (MIM 605535)/histone deacetylase (HDAC; see MIM 601241) complex (BHC) that mediates repression of neuron-specific genes through the cis-regulatory element known as repressor element-1 (RE1) or neural restrictive silencer (NRS) (Hakimi et al., 2002 [PubMed 12032298]).
References
Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010) :
Klajn, A., et al. J. Neurosci. 29(19):6296-6307(2009)
Hailesellasse Sene, K., et al. BMC Genomics 8, 85 (2007) :
Shi, Y.J., et al. Mol. Cell 19(6):857-864(2005)
Iwase, S., et al. Biochem. Biophys. Res. Commun. 322(2):601-608(2004)
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