|Application ||WB, E|
|Other Accession||Q6ZPK0, NP_057705.3|
|Calculated MW||74854 Da|
|Antigen Region||436-464 aa|
|Other Names||PHD finger protein 21A, BHC80a, BRAF35-HDAC complex protein BHC80, PHF21A, BHC80, KIAA1696|
|Target/Specificity||This PHF21A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 436-464 amino acids from the Central region of human PHF21A.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||PHF21A Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Component of the BHC complex, a corepressor complex that represses transcription of neuron-specific genes in non-neuronal cells. The BHC complex is recruited at RE1/NRSE sites by REST and acts by deacetylating and demethylating specific sites on histones, thereby acting as a chromatin modifier. In the BHC complex, it may act as a scaffold. Inhibits KDM1A-mediated demethylation of 'Lys-4' of histone H3 in vitro, suggesting a role in demethylation regulation.|
|Tissue Location||Highly expressed in brain. Expressed at much lower level in other tissues.|
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Provided below are standard protocols that you may find useful for product applications.
The PHF21A gene encodes BHC80, a component of a BRAF35 (MIM 605535)/histone deacetylase (HDAC; see MIM 601241) complex (BHC) that mediates repression of neuron-specific genes through the cis-regulatory element known as repressor element-1 (RE1) or neural restrictive silencer (NRS) (Hakimi et al., 2002 [PubMed 12032298]).
Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010) :
Klajn, A., et al. J. Neurosci. 29(19):6296-6307(2009)
Hailesellasse Sene, K., et al. BMC Genomics 8, 85 (2007) :
Shi, Y.J., et al. Mol. Cell 19(6):857-864(2005)
Iwase, S., et al. Biochem. Biophys. Res. Commun. 322(2):601-608(2004)
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