|Application ||WB, E|
|Calculated MW||55689 Da|
|Antigen Region||173-202 aa|
|Other Names||Zinc finger protein GLIS2, GLI-similar 2, Neuronal Krueppel-like protein, GLIS2, NKL|
|Target/Specificity||This GLIS2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 173-202 amino acids from the Central region of human GLIS2.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||GLIS2 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Can act either as a transcriptional repressor or as a transcriptional activator, depending on the cell context. Acts as a repressor of the Hedgehog signaling pathway (By similarity). Represses the Hedgehog-dependent expression of Wnt4 (By similarity). Necessary to maintain the differentiated epithelial phenotype in renal cells through the inhibition of SNAI1, which itself induces the epithelial-to-mesenchymal transition (By similarity). Represses transcriptional activation mediated by CTNNB1 in the Wnt signaling pathway. May act by recruiting the corepressors CTBP1 and HDAC3. May be involved in neuron differentiation (By similarity).|
|Cellular Location||Nucleus speckle. Cytoplasm|
|Tissue Location||Expressed at high levels in kidney and at low levels in heart, lung and placenta. Expressed in colon|
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Provided below are standard protocols that you may find useful for product applications.
This gene is a member of the GLI-similar zinc finger protein family and encodes a nuclear transcription factor with five C2H2-type zinc finger domains. The protein encoded by this gene is widely expressed at low levels in the neural tube and peripheral nervous system and likely promotes neuronal differentiation. It is abundantly expressed in the kidney and may have a role in the regulation of kidney morphogenesis. p120 regulates the expression level of this protein and induces the cleavage of this protein's C-terminal zinc finger domain. This protein also promotes the nuclear translocation of p120. Mutations in this gene cause nephronophthisis (NPHP), an autosomal recessive kidney disease characterized by tubular basement membrane disruption, interstitial lymphohistiocytic cell infiltration, and development of cysts at the corticomedullary border of the kidneys.
Attanasio, M., et al. Nat. Genet. 39(8):1018-1024(2007)
Hosking, C.R., et al. Mol. Biol. Cell 18(5):1918-1927(2007)
Kim, Y.S., et al. FEBS Lett. 581(5):858-864(2007)
Olsen, J.V., et al. Cell 127(3):635-648(2006)
Kim, Y.S., et al. Nucleic Acids Res. 31(19):5513-5525(2003)
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