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TSC22D2 Antibody (N-term)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • IF - TSC22D2 Antibody (N-term) AP16530A
    Fluorescent confocal image of A431 cell stained with TSC22D2 Antibody (N-term)(Cat#AP16530a).A431 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with TSC22D2 primary antibody (1:25, 1 h at 37℃). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37℃).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37℃). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min). TSC22D2 immunoreactivity is localized to Cytoplasm significantly.
    detail
  • WB - TSC22D2 Antibody (N-term) AP16530A
    TSC22D2 Antibody (N-term) (Cat. #AP16530a) western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the TSC22D2 antibody detected the TSC22D2 protein (arrow).
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
IF, WB, E
Primary Accession O75157
Other Accession NP_055594.1
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 79228 Da
Antigen Region 34-62 aa
Additional Information
Gene ID 9819
Other Names TSC22 domain family protein 2, TSC22-related-inducible leucine zipper protein 4, TSC22D2, KIAA0669, TILZ4
Target/Specificity This TSC22D2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 34-62 amino acids from the N-terminal region of human TSC22D2.
Dilution IF~~1:10~50
WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsTSC22D2 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name TSC22D2 (HGNC:29095)
Function Reduces the level of nuclear PKM isoform M2 which results in repression of cyclin CCND1 transcription and reduced cell growth.
Research Areas
Citations (0)
citation

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Background

TSC22D2 belongs to the TSC-22/Dip/Bun family and the function remains unknown.

References

Gluderer, S., et al. J. Biol. 9 (1), 9 (2010) :
Gevaert, K., et al. Nat. Biotechnol. 21(5):566-569(2003)
Gevaert, K., et al. Nat. Biotechnol. 21(5):566-569(2003)

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$ 182.50
$ 70.00
Cat# AP16530A
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