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ARNT2 Antibody (Center)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

  • WB - ARNT2 Antibody (Center) AP16544C
    ARNT2 Antibody (Center) (Cat. #AP16544c) western blot analysis in 293 cell line lysates (35ug/lane).This demonstrates the ARNT2 antibody detected the ARNT2 protein (arrow).
  • IF - ARNT2 Antibody (Center) AP16544C
    Fluorescent confocal image of SK-BR-3 cell stained with ARNT2 Antibody (Center)(Cat#AP16544c). SK-BR-3 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with ARNT2 primary antibody (1:25, 1 h at 37℃). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37℃).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37℃). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min).ARNT2 immunoreactivity is localized to nucleus significantly.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q9HBZ2
Other Accession Q78E60, Q61324, NP_055677.3
Reactivity Human
Predicted Mouse, Rat
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 78691 Da
Antigen Region 249-278 aa
Additional Information
Gene ID 9915
Other Names Aryl hydrocarbon receptor nuclear translocator 2, ARNT protein 2, Class E basic helix-loop-helix protein 1, bHLHe1, ARNT2, BHLHE1, KIAA0307
Target/Specificity This ARNT2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 249-278 amino acids from the Central region of human ARNT2.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsARNT2 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name ARNT2
Synonyms BHLHE1, KIAA0307
Function Transcription factor that plays a role in the development of the hypothalamo-pituitary axis, postnatal brain growth, and visual and renal function (PubMed:24022475). Specifically recognizes the xenobiotic response element (XRE).
Cellular Location Nucleus {ECO:0000255|PROSITE- ProRule:PRU00981}
Research Areas
Citations (0)

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This gene encodes a member of the basic-helix-loop-helix-Per-Arnt-Sim (bHLH-PAS) superfamily of transcription factors. The encoded protein acts as a partner for several sensor proteins of the bHLH-PAS family, forming heterodimers with the sensor proteins that bind regulatory DNA sequences in genes responsive to developmental and environmental stimuli. Under hypoxic conditions, the encoded protein complexes with hypoxia-inducible factor 1alpha in the nucleus and this complex binds to hypoxia-responsive elements in enhancers and promoters of oxygen-responsive genes. A highly similar protein in mouse forms functional complexes with both aryl hydrocarbon receptors and Single-minded proteins, suggesting addition roles for the encoded protein in the metabolism of xenobiotic compounds and the regulation of neurogenesis, respectively.


Ramirez, J.M., et al. Eur. J. Immunol. 40(9):2450-2459(2010)
Jugessur, A., et al. PLoS ONE 5 (7), E11493 (2010) :
Chakrabarti, B., et al. Autism Res 2(3):157-177(2009)
Ferreira, M.A., et al. Nat. Genet. 40(9):1056-1058(2008)
Martinez, V., et al. Breast Cancer Res. Treat. 110(3):521-530(2008)

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Cat# AP16544C
(40 western blots)
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